| Literature DB >> 16660412 |
Abstract
Proplastids from developing castor bean (Ricinus communis) endosperm have a pyruvate kinase activity which is extremely unstable on isolation from the organelle. It can be stabilized by 20 mm 2-mercaptoethanol in 20% ethylene glycol. In contrast the soluble pyruvate kinase is stable at 60 C for 10 minutes. The two activities have different pH optima. The soluble and the proplastid activities are eluted from a diethylaminoethyl-Sephadex A-25 sievorptive column at different ionic strengths.Entities:
Year: 1978 PMID: 16660412 PMCID: PMC1092036 DOI: 10.1104/pp.61.6.1037
Source DB: PubMed Journal: Plant Physiol ISSN: 0032-0889 Impact factor: 8.340