Literature DB >> 16660222

Change in the proportion of two aspartokinases in carrot root tissue in response to in vitro culture.

K Sakano1, A Komamine.   

Abstract

Two isofunctional aspartokinases (EC 2.7.2.4) exist in fresh root tissue of carrot (Daucus carota, cv. Oogata sanzun). The threoninesensitive portion constitutes about 70% of the activity; the lysinesensitive, less than 20%. Culture of slices of carrot tissue for 3 days reversed the ratio as the lysine-sensitive activity preferentially increased. Inhibition by threonine and lysine was additive in both enzyme preparations from fresh and cultured tissues. The activities were resolved into two distinct fractions of different sensitivity to threonine and lysine by DEAE-Sephadex A-50 column chromatography.

Entities:  

Year:  1978        PMID: 16660222      PMCID: PMC1091809          DOI: 10.1104/pp.61.1.115

Source DB:  PubMed          Journal:  Plant Physiol        ISSN: 0032-0889            Impact factor:   8.340


  6 in total

1.  Regulation by methionine of the synthesis of a third aspartokinase and of a second homoserine dehydrogenase in Escherichia coli K 12.

Authors:  J C Patte; G Le Bras; G N Cohen
Journal:  Biochim Biophys Acta       Date:  1967-03-22

2.  Sequence of enzyme synthesis and gene replication during the cell cycle of Bacillus subtilis.

Authors:  M Masters; A B Pardee
Journal:  Proc Natl Acad Sci U S A       Date:  1965-07       Impact factor: 11.205

3.  Isolation and characterization of a lysine-sensitive aspartokinase from a multicellular plant.

Authors:  P A Bryan; R D Cawley; C E Brunner; J K Bryan
Journal:  Biochem Biophys Res Commun       Date:  1970-12-09       Impact factor: 3.575

4.  Aspartokinase from wheat germ: isolation, characterization, and regulation.

Authors:  K F Wong; D T Dennis
Journal:  Plant Physiol       Date:  1973-02       Impact factor: 8.340

5.  Aspartokinase in Lemna minor L: Studies on the in Vivo and in Vitro Regulation of the Enzyme.

Authors:  K F Wong; D T Dennis
Journal:  Plant Physiol       Date:  1973-02       Impact factor: 8.340

6.  Properties and Regulation of Aspartate Kinase from Barley Seedlings (Hordeum vulgare L.).

Authors:  P R Shewry; B J Miflin
Journal:  Plant Physiol       Date:  1977-01       Impact factor: 8.340

  6 in total
  9 in total

1.  Regulation of lysine synthesis in transgenic potato plants expressing a bacterial dihydrodipicolinate synthase in their chloroplasts.

Authors:  A Perl; O Shaul; G Galili
Journal:  Plant Mol Biol       Date:  1992-08       Impact factor: 4.076

2.  Regulation of Lysine and Threonine Synthesis.

Authors:  G. Galili
Journal:  Plant Cell       Date:  1995-07       Impact factor: 11.277

3.  Regulation of lysine and threonine synthesis in carrot cell suspension cultures and whole carrot roots.

Authors:  B F Matthews; J M Widholm
Journal:  Planta       Date:  1978-01       Impact factor: 4.116

4.  The location of acid invertase activity and sucrose in the vacuoles of storage roots of beetroot (Beta vulgaris).

Authors:  R A Leigh; T Rees; W A Fuller; J Banfield
Journal:  Biochem J       Date:  1979-03-15       Impact factor: 3.857

5.  Derepression and Repression of Lysine-sensitive Aspartokinase during in Vitro Culture of Carrot Root Tissue.

Authors:  K Sakano
Journal:  Plant Physiol       Date:  1979-03       Impact factor: 8.340

6.  Bifunctional protein in carrot contains both aspartokinase and homoserine dehydrogenase activities.

Authors:  B J Wilson; A C Gray; B F Matthews
Journal:  Plant Physiol       Date:  1991-12       Impact factor: 8.340

7.  Characterization of cultured tobacco cell lines resistant to ethionine, a methionine analog.

Authors:  R A Gonzales; P K Das; J M Widholm
Journal:  Plant Physiol       Date:  1984-03       Impact factor: 8.340

8.  Threonine accumulation in the seeds of a barley mutant with an altered aspartate kinase.

Authors:  S W Bright; B J Miflin; S E Rognes
Journal:  Biochem Genet       Date:  1982-04       Impact factor: 1.890

9.  Feedback-insensitive aspartate kinase isoenzymes in barley mutants resistant to lysine plus threonine.

Authors:  S E Rognes; S W Bright; B J Miflin
Journal:  Planta       Date:  1983-02       Impact factor: 4.116

  9 in total

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