| Literature DB >> 16660222 |
Abstract
Two isofunctional aspartokinases (EC 2.7.2.4) exist in fresh root tissue of carrot (Daucus carota, cv. Oogata sanzun). The threoninesensitive portion constitutes about 70% of the activity; the lysinesensitive, less than 20%. Culture of slices of carrot tissue for 3 days reversed the ratio as the lysine-sensitive activity preferentially increased. Inhibition by threonine and lysine was additive in both enzyme preparations from fresh and cultured tissues. The activities were resolved into two distinct fractions of different sensitivity to threonine and lysine by DEAE-Sephadex A-50 column chromatography.Entities:
Year: 1978 PMID: 16660222 PMCID: PMC1091809 DOI: 10.1104/pp.61.1.115
Source DB: PubMed Journal: Plant Physiol ISSN: 0032-0889 Impact factor: 8.340