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Literature DB >> 16660151

Isolation and oxidative properties of intact mitochondria isolated from spinach leaves.

Abstract

A procedure was described for preparing intact mitochondria from spinach (Spinacia oleracea L.) leaves. These mitochondria oxidized succinate, malate, pyruvate, alpha-ketoglutarate, and NADH with good respiratory control and ADP/O ratios comparable to those observed with mitochondria from other plant tissues. Glycine was oxidized by the preparations. This oxidation linked to the mitochondrial electron transport chain, was coupled to three phosphorylation sites and was sensitive to electron transport and phosphorylation inhibitors.Cyanide completely inhibited the oxidation of NADH. The oxidation of succinate, malate, and glycine was only partially inhibited.

Entities: Chemical Species

Year: 1977 PMID： 16660151 PMCID： PMC542677 DOI： 10.1104/pp.60.4.625

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

8 in total

Review 1. Cyanide-insensitive respiration. An alternative mitochondrial pathway.

Authors: M F Henry; E D Nyns
Journal: Subcell Biochem Date: 1975-03

2. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors: D I Arnon
Journal: Plant Physiol Date: 1949-01 Impact factor: 8.340

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. The oxidation of malate by isolated plant mitochondria.

Authors: J O Coleman; J M Palmer
Journal: Eur J Biochem Date: 1972-04-24

5. Adenosine triphosphate synthesis and the natural electron acceptor for synthesis of serine from glycine in leaves.

Authors: I F Bird; M J Cornelius; A J Keys; C P Whittingham
Journal: Biochem J Date: 1972-06 Impact factor: 3.857

6. Oxidative phosphorylation during glycollate metabolism in mitochondria from phototrophic Euglena gracilis.

Authors: N Collins; R H Brown; M J Merrett
Journal: Biochem J Date: 1975-09 Impact factor: 3.857

7. Metabolic processes in cytoplasmic particles of the avocado fruit. IX. The oxidation of pyruvate and malate during the climacteric cycle.

Authors: C Lance; G E Hobson; R E Young; J B Biale
Journal: Plant Physiol Date: 1967-04 Impact factor: 8.340

8. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids.

Authors: G R Schonbaum; W D Bonner; B T Storey; J T Bahr
Journal: Plant Physiol Date: 1971-01 Impact factor: 8.340

8 in total

47 in total

1. Cloning of the gene (gdcH) encoding H-protein, a component of the glycine decarboxylase complex of pea (Pisum sativum L.).

Authors: D Macherel; J Bourguignon; R Douce
Journal: Biochem J Date: 1992-09-01 Impact factor: 3.857

Isolation and oxidative properties of intact mitochondria isolated from spinach leaves.

Review 1. Cyanide-insensitive respiration. An alternative mitochondrial pathway.

2. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

3. Protein measurement with the Folin phenol reagent.

4. The oxidation of malate by isolated plant mitochondria.

5. Adenosine triphosphate synthesis and the natural electron acceptor for synthesis of serine from glycine in leaves.

6. Oxidative phosphorylation during glycollate metabolism in mitochondria from phototrophic Euglena gracilis.

7. Metabolic processes in cytoplasmic particles of the avocado fruit. IX. The oxidation of pyruvate and malate during the climacteric cycle.

8. Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids.

1. Cloning of the gene (gdcH) encoding H-protein, a component of the glycine decarboxylase complex of pea (Pisum sativum L.).

2. Focus Issue on Metabolism: Metabolites, Metabolites Everywhere.

3. The re-assimilation of ammonia produced by photorespiration and the nitrogen economy of C3 higher plants.

4. Properties of substantially chlorophyll-free pea leaf mitochondria prepared by sucrose density gradient separation.

5. Properties and intramitochondrial localization of serine hydroxymethyltransferase in leaves of higher plants.

6. Studies on the aminotransferases participating in the glycolate metabolism of the alga mougeotia.

7. Simultaneous oxidation of glycine and malate by pea leaf mitochondria.

8. Separation of mitochondria from contaminating subcellular structures utilizing silica sol gradient centrifugation.

9. Do angiosperms with highly divergent mitochondrial genomes have altered mitochondrial function?

10. Nuclear and mitochondrial DNA have sequence homology with a chloroplast gene.