Literature DB >> 16660054

A rapid, simple method for nuclei isolation from plant protoplasts.

K Ohyama1, L E Pelcher, D Horn.   

Abstract

A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.

Entities:  

Year:  1977        PMID： 16660054      PMCID： PMC542574          DOI： 10.1104/pp.60.2.179

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  4 in total

1.  DNA replication in isolated HeLa cell nuclei.

Authors:  O Bernard; T P Brent
Journal:  Biochem Biophys Res Commun       Date:  1973-08-21       Impact factor: 3.575

2.  Isolation and Properties of Nuclei from Control and Auxin-treated Soybean Hypocotyl.

Authors:  Y M Chen; C Y Lin; H Chang; T J Guilfoyle; J L Key
Journal:  Plant Physiol       Date:  1975-07       Impact factor: 8.340

3.  Isolation and Properties of Deoxyribonucleic Acid from Protoplasts of Cell Suspension Cultures of Ammi visnaga and Carrot (Daucus carota).

Authors:  K Ohyama; O L Gamborg; R A Miller
Journal:  Plant Physiol       Date:  1972-09       Impact factor: 8.340

4.  The effects of amino acids and ammonium on the growth of plant cells in suspension culture.

Authors:  O L Gamborg
Journal:  Plant Physiol       Date:  1970-04       Impact factor: 8.340
  4 in total
  7 in total

1.  Auxin reduces the synthesis of major vacuolar proteins in tobacco mesophyl protoplast.

Authors:  Y Meyer; Y Chartier; G Alibert
Journal:  Plant Physiol       Date:  1987-03       Impact factor: 8.340

2.  Investigations on the transfer of isolated nuclei into plant protoplasts.

Authors:  H Lörz; I Potrykus
Journal:  Theor Appl Genet       Date:  1978-11       Impact factor: 5.699

3.  Transcriptional regulation and DNA methylation of nuclear genes for photosynthesis in nongreen plant cells.

Authors:  J Ngernprasirtsiri; H Kobayashi; T Akazawa
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

4.  DNA Binding and Uptake by Nuclei Isolated from Plant Protoplasts: Fate of Single-stranded Bacteriophage fd DNA.

Authors:  K Ohyama
Journal:  Plant Physiol       Date:  1978-04       Impact factor: 8.340

5.  Isolation of intact plastids from protoplasts from castor bean endosperm.

Authors:  M Nishimura; H Beevers
Journal:  Plant Physiol       Date:  1978-07       Impact factor: 8.340

6.  Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families.

Authors:  Sidona Sikorskaite; Minna-Liisa Rajamäki; Danas Baniulis; Vidmantas Stanys; Jari Pt Valkonen
Journal:  Plant Methods       Date:  2013-07-26       Impact factor: 4.993

7.  A simple plant high-molecular-weight DNA extraction method suitable for single-molecule technologies.

Authors:  Zhigang Li; Stephen Parris; Christopher A Saski
Journal:  Plant Methods       Date:  2020-03-14       Impact factor: 4.993
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.