| Literature DB >> 16660017 |
M Neuburger1, J Joyard, R Douce.
Abstract
Yeast cationic ferricytochrome c was able to bind to the spinach (Spinacia oleracea) chloroplast envelope with a low affinity (Kd = 1.1 mum). The total amount of low affinity binding sites was of the order of 50 nmol cytochrome c mg(-1) protein. We gave the evidence that binding of ferricytochrome c to the envelope was electrostatic and that the envelope membranes were strongly negatively charged. Addition of yeast ferricytochrome c to a preparation of intact washed chloroplasts (class I) induced a strong agglutination of chloroplasts.Entities:
Year: 1977 PMID: 16660017 PMCID: PMC542530 DOI: 10.1104/pp.59.6.1178
Source DB: PubMed Journal: Plant Physiol ISSN: 0032-0889 Impact factor: 8.340