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Literature DB >> 16659880

Growth of Suspension-cultured Acer pseudoplatanus L. Cells in Automatic Culture Units of Large Volume.

Abstract

A phytostat to mass culture higher plant cells in liquid medium is described. This apparatus allowed the culture in batch, turbidostat and chemostat of 20 liters of cells. Automatic control of cell suspension growth was based on culture turbidity. Changes with time of certain cell characteristics, particularly cell respiration and phospholipid content, indicated that the test time to harvest large amounts of sycamore cells (Acer pseudoplatanus L.) in good physiological state was about 2 days before the end of the exponential phase of growth, when the cell density reached one million cells per milliliter of culture.

Entities: Chemical Species

Year: 1977 PMID： 16659880 PMCID： PMC542431 DOI： 10.1104/pp.59.3.502

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

7 in total

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Journal: Exp Cell Res Date: 1964-01 Impact factor: 3.905

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Journal: Plant Physiol Date: 1961-07 Impact factor: 8.340

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Journal: Plant Physiol Date: 1976-03 Impact factor: 8.340

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Authors: J J Leguay; J Guern
Journal: Plant Physiol Date: 1975-09 Impact factor: 8.340

7 in total

23 in total

1. Regulation of tonoplast k channels by voltage in the range of physiological electric potentials.

Authors: R Colombo; R Cerana; P Lado
Journal: Plant Physiol Date: 1990-05 Impact factor: 8.340

2. Characterization of a cytosolic aconitase in higher plant cells.

Authors: R Brouquisse; M Nishimura; J Gaillard; R Douce
Journal: Plant Physiol Date: 1987-08 Impact factor: 8.340

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Authors: C Alban; J Joyard; R Douce
Journal: Plant Physiol Date: 1988-11 Impact factor: 8.340

4. Cytoplasmic pH Regulation in Acer pseudoplatanus Cells: II. Possible Mechanisms Involved in pH Regulation during Acid-Load.

Authors: Y Mathieu; J Guern; M Pean; C Pasquier; J C Beloeil; J Y Lallemand
Journal: Plant Physiol Date: 1986-11 Impact factor: 8.340

10. Origin of the cytoplasmic pH changes during anaerobic stress in higher plant cells. Carbon-13 and phosphorous-31 nuclear magnetic resonance studies.

Authors: E Gout; A Boisson; S Aubert; R Douce; R Bligny
Journal: Plant Physiol Date: 2001-02 Impact factor: 8.340