| Literature DB >> 16659663 |
Abstract
NADP-dependent glutamate dehydrogenase was partially purified from extracts of the marine siphonous green alga Caulerpa simpliciuscula. The enzyme had an apparent Km NH(4) (+) of 0.4 to 0.7 mm and was highly specific for NADPH, alpha-ketoglutarate, and ammonium ions.The bulk of the NADP-glutamate dehydrogenase was isolated with the chloroplast fraction in cell-free preparations of this alga and was released from these "chloroplast fractions" as a soluble enzyme on gentle lysis of chloroplast membranes.Entities:
Year: 1976 PMID: 16659663 PMCID: PMC542231 DOI: 10.1104/pp.58.3.283
Source DB: PubMed Journal: Plant Physiol ISSN: 0032-0889 Impact factor: 8.340