Literature DB >> 16659624

In vitro iodination of plant ribonucleic acids.

R R Meeker1, J R Thomas, K K Tewari.   

Abstract

The optimum conditions for in vitro iodination of RNAs have been established which yield specific radioactivities ranging from 10 x 10(4) to 10 x 10(6) cpm/mug. A nomogram has been constructed by correlating specific radioactivities of RNA with concentration of KI, RNA, and (125)I. This nomogram can be used to determine the conditions for the desired specific radioactivities for any unknown RNA. The in vitro iodinated RNA has been compared with in vivo labeled RNA for hybridization characteristics. Competition hybridization between (125)I-labeled chloroplast-rRNA and unlabeled pea (Pisum sativum) chloroplast-rRNA was identical to that found using [(32)P]chloroplast-rRNA. Thermal stability of DNA-(125)I-rRNA hybrids was similar to the thermal stability of DNA-[(32)P]rRNA hybrids. The iodinated RNA was not found to have undergone any changes in its hydrogen-bonding properties.

Entities:  

Year:  1976        PMID： 16659624      PMCID： PMC542182          DOI： 10.1104/pp.58.1.71

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  14 in total

1.  RNA-RNA hybridisation using 125I-labelled RNA from tobacco necrosis virus and its satellite.

Authors:  A Shoulder; G Darby; T Minson
Journal:  Nature       Date:  1974-10-25       Impact factor: 49.962

2.  Ribosomal-RNA genes in the chloroplast DNA of pea leaves.

Authors:  J R Thomas; K K Tewari
Journal:  Biochim Biophys Acta       Date:  1974-08-15

3.  Cyclodromes and palindromes in chromosomes.

Authors:  C A Thomas; R E Pyeritz; D A Wilson; B M Dancis; C S Lee; M D Bick; H L Huang; B H Zimm
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

4.  Hybridization of RNA labelled with 125 I to high specific activity.

Authors:  N H Scherberg; S Refetoff
Journal:  Nat New Biol       Date:  1973-04-04

5.  Iodination of ribosomal RNA in vitro.

Authors:  H J Heiniger; H W Chen; S L Commerford
Journal:  Int J Appl Radiat Isot       Date:  1973-07

6.  Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria.

Authors:  R L Moore; B J McCarthy
Journal:  J Bacteriol       Date:  1967-10       Impact factor: 3.490

7.  The introduction of radioisotopes into RNA by methylation in vitro.

Authors:  K D Smith; J L Armstrong; B J McCarthy
Journal:  Biochim Biophys Acta       Date:  1967-07-18

8.  General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus.

Authors:  E H Davidson; B R Hough; C S Amenson; R J Britten
Journal:  J Mol Biol       Date:  1973-06-15       Impact factor: 5.469

9.  The use of RNA labeled in vitro with iodine-125 in molecular hybridization experiments.

Authors:  M J Getz; L C Altenburg; G F Saunders
Journal:  Biochim Biophys Acta       Date:  1972-12-22

10.  A proposal for the structure of the Drosophila genome.

Authors:  J Bonner; J R Wu
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205
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  4 in total

1.  Mapping of rRNA genes in an inverted repeat in Nicotiana tabacum chloroplast DNA.

Authors:  J E Jurgenson; D P Bourque
Journal:  Nucleic Acids Res       Date:  1980-08-25       Impact factor: 16.971

2.  Comparison between the organization of nuclear ribosomal DNA unit of Euglena gracilis Z and var. Bacillaris.

Authors:  O Neyret-Djossou; G Freyssinet; P Ravel-Chapuis; P Heizmann
Journal:  Plant Mol Biol       Date:  1986-03       Impact factor: 4.076

3.  Quantitative estimations of chloroplast DNA in bleached mutants of Euglena gracilis.

Authors:  Y Hussein; P Heizmann; P Nicolas; V Nigon
Journal:  Curr Genet       Date:  1982-11       Impact factor: 3.886

4.  Modifications of chloroplast DNA during streptomycin induced mutagenesis in Euglena gracilis.

Authors:  P Heizmann; Y Hussein; P Nicolas; V Nigon
Journal:  Curr Genet       Date:  1982-05       Impact factor: 3.886
  4 in total

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