Literature DB >> 16659496

Influence of the nutrient medium on the recovery of dividing cells from tobacco protoplasts.

H Uchimiya1, T Murashige.   

Abstract

Systematic tests resulted in a nutrient solution containing the following, in milligrams per liter, for the culture of protoplasts isolated from Nicotiana tabacum L. callus cells: Murashige and Skoog salts (T. Murashige and F. Skoog, 1962. Physiol. Plant. 15: 473-497); sucrose, 15,000; mannitol, 110,000; alpha-naphthaleneacetic acid, 0.6; kinetin, 0-0.1; thiamine.HCl, 10; pyridoxine.HCl, 10; nicotinic acid, 5; myo-inositol, 100; and glycine, 2. In this medium, regeneration of cell wall has been observed in 85% and resumption of cell division among 35% of the protoplast isolates.

Entities:  

Year:  1976        PMID： 16659496      PMCID： PMC542039          DOI： 10.1104/pp.57.3.424

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  5 in total

1.  Further observations on acetylcholine noise.

Authors:  B Katz; R Miledi
Journal:  Nat New Biol       Date:  1971-07-28

2.  Regeneration of tobacco and petunia plants from protoplasts and culture of corn protoplasts.

Authors:  V Vasil; I K Vasil
Journal:  In Vitro       Date:  1974 Jul-Aug

3.  Callus formation from single protoplasts of petunia.

Authors:  I Potrykus; J Durand
Journal:  Nat New Biol       Date:  1972-06-28

4.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

5.  Cell division in newly formed cells from protoplasts of soybean.

Authors:  K N Kao; W A Keller; R A Miller
Journal:  Exp Cell Res       Date:  1970-10       Impact factor: 3.905
  5 in total
  8 in total

1.  Callus formation from protoplasts of culturedSpinacia oleracea cells.

Authors:  H Nakagawa; H Tanaka; T Oba; N Ogura; M Iizuka
Journal:  Plant Cell Rep       Date:  1985-05       Impact factor: 4.570

2.  Callus formation from protoplasts of cultured Lithospermum erythrorhizon cells.

Authors:  Y Maeda; Y Fujita; Y Yamada
Journal:  Plant Cell Rep       Date:  1983-08       Impact factor: 4.570

3.  Monoclonal antibodies to plant plasma-membrane antigens.

Authors:  P M Norman; V P Wingate; M S Fitter; C J Lamb
Journal:  Planta       Date:  1986-04       Impact factor: 4.116

4.  Pathway of Salicylic Acid Biosynthesis in Healthy and Virus-Inoculated Tobacco.

Authors:  N. Yalpani; J. Leon; M. A. Lawton; I. Raskin
Journal:  Plant Physiol       Date:  1993-10       Impact factor: 8.340

5.  Quantitative analysis of the fate of exogenous DNA in Nicotiana protoplasts.

Authors:  H Uchimiya; T Murashige
Journal:  Plant Physiol       Date:  1977-02       Impact factor: 8.340

6.  Salicylic Acid induces cyanide-resistant respiration in tobacco cell-suspension cultures.

Authors:  Y Kapulnik; N Yalpani; I Raskin
Journal:  Plant Physiol       Date:  1992-12       Impact factor: 8.340

7.  Microcallus growth from maize protoplasts.

Authors:  C Imbrie-Milligan; K K Kamo; T K Hodges
Journal:  Planta       Date:  1987-05       Impact factor: 4.116

8.  The isolation and culture of protoplasts from an embryogenic cell suspension culture of Picea glauca (moench) voss.

Authors:  F Bekkaoui; P K Saxena; S M Attree; L C Fowke; D I Dunstan
Journal:  Plant Cell Rep       Date:  1987-12       Impact factor: 4.570
  8 in total

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