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Literature DB >> 16659409

Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl.

C Y Lin¹, T J Guilfoyle, Y M Chen, J L Key.

Abstract

An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the alpha-amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I.

Entities: Chemical Species

Year: 1975 PMID： 16659409 PMCID： PMC541939 DOI： 10.1104/pp.56.6.850

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

19 in total

1. Histone, a suppressor of chromosomal RNA synthesis.

Authors: R C HUANG; J BONNER
Journal: Proc Natl Acad Sci U S A Date: 1962-07-15 Impact factor: 11.205

2. RNA polymerase from eukaryotic cells. Isolation and purification of enzymes and factors from chromatin of coconut nuclei.

Authors: H Mondal; R K Mandal; B B Biswas
Journal: Eur J Biochem Date: 1972-02

3. Isolation and partial characterization of the multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in the mouse myeloma, MOPC 315.

Authors: L B Schwartz; V E Sklar; J A Jaehning; R Weinmann; R G Roeder
Journal: J Biol Chem Date: 1974-09-25 Impact factor: 5.157

Isolation of nucleoli and localization of ribonucleic Acid polymerase I from soybean hypocotyl.

1. Histone, a suppressor of chromosomal RNA synthesis.

2. RNA polymerase from eukaryotic cells. Isolation and purification of enzymes and factors from chromatin of coconut nuclei.

3. Isolation and partial characterization of the multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in the mouse myeloma, MOPC 315.

4. Products of RNA polymerases in HeLa cell nuclei.

5. The separation of RNA polymerases I and II achieved by fractionation of plant chromatin.

6. Isolation, purification and characterization of RNA polymerases from wheat germ.

7. The DNA-directed RNA polymerases of soybean.

8. RNA polymerase 3 of Blastocladiella emersonii is mitochondrial.

9. Isolation and Properties of Nuclei from Control and Auxin-treated Soybean Hypocotyl.

10. Separation and partial characterization of multiple ribonucleic Acid polymerases from soybean hypocotyl.

1. Ribonucleic acid synthesis and loss of viability in pea seed.

2. Fractionation of nucleoli from auxin-treated soybean hypocotyl into nucleolar chromatin and preribosomal particles.

3. Selective Modulation of RNA Polymerase I Activity during Growth Transitions in the Soybean Seedling.

4. Purification and Characterization of DNA-dependent RNA Polymerases from Cauliflower Nuclei.