Literature DB >> 16659402

A rapid technique for the estimation of polynucleotide adenylyltransferase and ribonucleic Acid polymerase in plant tissues.

T J Walter1, R J Mans.   

Abstract

Nucleic acid-dependent polynucleotide adenylytransferase (EC 2.7.7.19) and ribonucleic acid polymerase (EC 2.7.7.6) have been partially purified from maize tissues (Zea mays L.) utilizing ammonium sulfate precipitation and batch diethylaminoethylcellulose chromatography. The technique is applicable to the simultaneous processing of up to eight samples of plant tissue and affords a rapid and reproducible means of assaying these two enzymes from small quantities of kernels or seedlings. The kinetic characteristics of the partially purified enzymes resemble those from more extensively purified preparations.

Entities:  

Year:  1975        PMID： 16659402      PMCID： PMC541932          DOI： 10.1104/pp.56.6.821

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  23 in total

1.  Utilization of ribonucleic acid and deoxyoligomer primers for polyadenylic acid synthesis by adenosine triphosphate: polynucleotidylexotransferase from maize.

Authors:  R J Mans; N J Huff
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

2.  Poly(A) sequences in plant polysomal RNA.

Authors:  T J Higgins; J F Mercer; P B Goodwin
Journal:  Nat New Biol       Date:  1973-11-21

3.  Polyadenylic sequences in plant RNA.

Authors:  C Van de Walle
Journal:  FEBS Lett       Date:  1973-08-01       Impact factor: 4.124

4.  Rifampicin inhibition of poly (dT)-poly(A)-primed poly (A) synthesis by HeLa AMP polynucleotidylexotransferase.

Authors:  R J Mans
Journal:  FEBS Lett       Date:  1973-07-01       Impact factor: 4.124

5.  Addition of polyadenylic acid to RNA by ATP: polynucleotidylexotransferase partially purified from HeLa cells.

Authors:  R Mans; G Stein
Journal:  Life Sci       Date:  1974-02-01       Impact factor: 5.037

6.  The presence of polyadenylate sequences in the ribonucleic acid of a higher plant.

Authors:  C O Manahan; A A App; C C Still
Journal:  Biochem Biophys Res Commun       Date:  1973-07-17       Impact factor: 3.575

7.  A poly(A) polymerase from calf thymus. Purification and properities of the enzyme.

Authors:  M A Winters; M Edmonds
Journal:  J Biol Chem       Date:  1973-07-10       Impact factor: 5.157

8.  Terminal riboadenylate transferase: a polyadenylate polymerase from calf thymus gland.

Authors:  C M Tsiapalis; J W Dorson; D M De Sante; F J Bollum
Journal:  Biochem Biophys Res Commun       Date:  1973-02-05       Impact factor: 3.575

9.  Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue.

Authors:  J V Jacobsen; J A Zwar
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205

10.  The genome of RNA tumor viruses contains polyadenylic acid sequences.

Authors:  M Green; M Cartas
Journal:  Proc Natl Acad Sci U S A       Date:  1972-04       Impact factor: 11.205
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  1 in total

1.  Adenylate metabolism of embryonic axes from deteriorated soybean seeds.

Authors:  J D Anderson
Journal:  Plant Physiol       Date:  1977-04       Impact factor: 8.340
  1 in total

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