Literature DB >> 16659395

Purification of myo-Inositol 1-Phosphate Synthase from Rice Cell Culture by Affinity Chromatography.

E A Funkhouser1, F A Loewus.   

Abstract

myo-Inositol 1-phosphate synthase (EC 5.5.1.4) is the enzyme which catalyzes the synthesis of the precursor for the myo-inositol oxidation pathway. Rice callus grown in suspension culture provides a good source of plant enzyme. Use has been made of a noncompetitive inhibitor to prepare an affinity column for this enzyme. With this column, the enzyme from rice callus has been purified 1500-fold in a single step, about 9000-fold over-all, to a specific activity of 0.078 units per milligram of protein. This is an order of magnitude greater than previous purifications of the plant enzyme.

Entities:  

Year:  1975        PMID： 16659395      PMCID： PMC541925          DOI： 10.1104/pp.56.6.786

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  19 in total

1.  BIOCHEMICAL STUDIES ON INOSITOL. VII. BIOSYNTHESIS OF INOSITOL BY A SOLUBLE ENZYME SYSTEM.

Authors:  I W CHEN; F C CHARALAMPOUS
Journal:  J Biol Chem       Date:  1964-06       Impact factor: 5.157

2.  [STUDIES ON THE BIOSYNTHESIS OF CYCLITOLS. II. FORMATION OF MESO-INOSITOL FROM C14-1-GLUCOSE IN SINAPIS ALBA AND SELECTIVE DEGRADATION OF THE RESULTING PRODUCT].

Authors:  H KINDL; O HOFFMANN-OSTENHOF
Journal:  Biochem Z       Date:  1964-03-12

3.  A MEMBRANE METHOD FOR DETERMINATION OF TOTAL PROTEIN IN DILUTE ALGAL SUSPENSIONS.

Authors:  C A PRICE
Journal:  Anal Biochem       Date:  1965-08       Impact factor: 3.365

4.  Growth of Rice Root-derived Callus Tissue in Suspension Culture.

Authors:  H B Lieb; T B Ray; C C Still
Journal:  Plant Physiol       Date:  1973-06       Impact factor: 8.340

5.  Studies on the biosynthesis of cyclitols, XXX. Purification of myo-inositol-1-phosphate synthase of rat testes to homogeneity by affinity chromatography on NAD-sepharose.

Authors:  F Pittner; W Fried; O Hoffmann-Ostenhof
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1974-02

6.  Biosynthesis of myo-inositol in rat mammary gland. Isolation and properties of the enzymes.

Authors:  W F Naccarato; R E Ray; W W Wells
Journal:  Arch Biochem Biophys       Date:  1974-09       Impact factor: 4.013

7.  Mass spectrometric study on the mechanism of D-glucose 6-phosphate-L-myo-inositol 1-phosphate cyclase.

Authors:  W R Sherman; M A Stewart; M Zinbo
Journal:  J Biol Chem       Date:  1969-10-25       Impact factor: 5.157

8.  Inositol biosynthesis in Neurospora crassa.

Authors:  E Piña; E L Tatum
Journal:  Biochim Biophys Acta       Date:  1967-03-22

9.  The stereospecificity of D-glucose-6-phosphate: 1L-myo-inositol-1-phosphate cycloaldolase on the hydrogen atoms at C-6.

Authors:  S M Byun; R Jenness; W P Ridley; S Kirkwood
Journal:  Biochem Biophys Res Commun       Date:  1973-10-01       Impact factor: 3.575

10.  myo-Inositol 1-Phosphate Synthase Inhibition and Control of Uridine Diphosphate-d-glucuronic Acid Biosynthesis in Plants.

Authors:  M W Loewus; F Loewus
Journal:  Plant Physiol       Date:  1974-09       Impact factor: 8.340
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  4 in total

1.  L-myo-lnositol 1-Phosphate Synthase from Plant Sources (Characteristics of the Chloroplastic and Cytosolic Enzymes).

Authors:  A. RayChaudhuri; N. C. Hait; S. Dasgupta; T. J. Bhaduri; R. Deb; A. L. Majumder
Journal:  Plant Physiol       Date:  1997-10       Impact factor: 8.340

2.  Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division.

Authors:  M Biffen; D E Hanke
Journal:  Biochem J       Date:  1990-02-01       Impact factor: 3.857

3.  Comparative Proteomic and Physiological Analysis Reveals the Variation Mechanisms of Leaf Coloration and Carbon Fixation in a Xantha Mutant of Ginkgo biloba L.

Authors:  Xinliang Liu; Wanwen Yu; Guibin Wang; Fuliang Cao; Jinfeng Cai; Huanli Wang
Journal:  Int J Mol Sci       Date:  2016-10-27       Impact factor: 5.923

Review 4.  Inositol Pyrophosphate Pathways and Mechanisms: What Can We Learn from Plants?

Authors:  Caitlin Cridland; Glenda Gillaspy
Journal:  Molecules       Date:  2020-06-17       Impact factor: 4.411
  4 in total

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