The Effect of Exogenous Nicotinamide Adenine Dinucleotide on the Oxidation of Nicotinamide Adenine Dinucleotide-linked Substrates by Isolated Plant Mitochondria.

The oxidation of malate, citrate, and alpha-ketoglutarate by cauliflower (Brassica oleacea L.) bud mitochondria was inhibited by rotenone. This inhibition was relieved upon addition of NAD(+) to the medium, and ADP/O values were lowered to less than 2 when both rotenone and NAD(+) were present. Dinitrophenol did not affect the relief of rotenone inhibition by exogenous NAD(+).