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Literature DB >> 16658722

Characteristics of an Acid protease from maize endosperm.

Abstract

An assay has been developed to measure protease activity in endosperm extracts of maize seeds. With hemoglobin as substrate, the enzyme(s) has a pH optimum of 3.8 and a temperature optimum of 46 C. It also degrades gliadin, edestin, bovine serum albumin, and partially hydrolyzed zein and glutelin under standard assay conditions. The enzyme(s) has endopeptidase activity with all substrates tested. When undenatured zein and glutelin are suspended in an agar gel, both are efficiently degraded. Using this assay, the protease activity increases from day 3 to day 8 after inhibition and then declines.

Entities: Gene Species

Year: 1974 PMID： 16658722 PMCID： PMC543247 DOI： 10.1104/pp.53.3.449

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

4 in total

16 in total

5. Purification and Characterization of Two Benzoyl-l-Tyrosine p-Nitroanilide Hydrolases from Etiolated Leaves of Zea mays L.

Authors: M Doi; Y Shioi
Journal: Plant Physiol Date: 1987-07 Impact factor: 8.340

Characteristics of an Acid protease from maize endosperm.

1. Protein measurement with the Folin phenol reagent.

2. The Hydrolysis of Endosperm Protein in Zea mays.

3. Gibberellic Acid-induced synthesis of protease by isolated aleurone layers of barley.

4. Enzymes associated with protein bodies isolated from ungerminated barley seeds.

1. Proteases of senescing oat leaves: I. Purification and general properties.

2. In Vitro Studies of Nitrate Reductase Activity in Cotton Cotyledons: Effects of Dowex 1-Cl and BSA.

3. Control of storage protein metabolism in the cotyledons of germinating mung beans: role of endopeptidase.

4. Globulin-specific Proteolytic Activity in Germinating Pumpkin Seeds as Detected by a Fluorescence Assay Method.

5. Purification and Characterization of Two Benzoyl-l-Tyrosine p-Nitroanilide Hydrolases from Etiolated Leaves of Zea mays L.

6. Storage-protein hydrolysis and protein-body breakdown in germinatedZea mays L. seeds.

7. Proteolytic activity in relationship to senescence and cotyledonary development in Pisum sativum L.

8. Development of Endopeptidase Activities in Maize (Zea mays L.) Endosperms.

9. The Hydrolysis of Endosperm Protein in Zea mays.

10. Leaf Proteolytic Activities and Senescence during Grain Development of Field-grown Corn (Zea mays L.).