Literature DB >> 16658375

Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa.

R H Schloemer1, R H Garrett.   

Abstract

The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO(3) (-) in the NADH-nitrate reductase assay are 10 mum, 2.6 mum, and 0.23 mm, respectively. Heat treatment exerts varying effects on the enzymatic activities associated with the nitrate reductase complex.

Entities:  

Year:  1973        PMID： 16658375      PMCID： PMC366311          DOI： 10.1104/pp.51.3.591

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  9 in total

1.  Mechanism of action of nitrate reductase from Neurospora.

Authors:  D J NICHOLAS; A NASON
Journal:  J Biol Chem       Date:  1954-11       Impact factor: 5.157

2.  Role of Molybdenum as a Constituent of Nitrate Reductase from Soybean Leaves.

Authors:  D J Nicholas; A Nason
Journal:  Plant Physiol       Date:  1955-03       Impact factor: 8.340

3.  Enzymic Assimilation of Nitrate in Tomato Plants. I. Reduction of Nitrate to Nitrite.

Authors:  G W Sanderson; E C Cocking
Journal:  Plant Physiol       Date:  1964-05       Impact factor: 8.340

4.  Pyridine Nucleotide-Nitrate Reductase from Extracts of Higher Plants.

Authors:  H J Evans; A Nason
Journal:  Plant Physiol       Date:  1953-04       Impact factor: 8.340

5.  Inactivation and repression by ammonium of the nitrate reducing system in chlorella.

Authors:  M Losada; A Paneque; P J Aparicio; J M Vega; J Cárdenas; J Herrera
Journal:  Biochem Biophys Res Commun       Date:  1970-03-27       Impact factor: 3.575

6.  Mechanism of nitrate reduction in Chlorella.

Authors:  W G Zumft; A Paneque; P J Aparicio; M Losada
Journal:  Biochem Biophys Res Commun       Date:  1969-09-10       Impact factor: 3.575

7.  Involvement of a B-type cytochrome in the assimilatory nitrate reductase of Neurospora crassa.

Authors:  R H Garrett; A Nason
Journal:  Proc Natl Acad Sci U S A       Date:  1967-10       Impact factor: 11.205

8.  Further purification and properties of Neurospora nitrate reductase.

Authors:  R H Garrett; A Nason
Journal:  J Biol Chem       Date:  1969-06-10       Impact factor: 5.157

9.  Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.

Authors:  K R Yamamoto; B M Alberts; R Benzinger; L Lawhorne; G Treiber
Journal:  Virology       Date:  1970-03       Impact factor: 3.616
  9 in total
  2 in total

1.  Nitrate uptake and nitrate reduction in synchronous Chlorella.

Authors:  R Tischner; H Lorenzen
Journal:  Planta       Date:  1979-01       Impact factor: 4.116

2.  Purification and Characterization of the Nitrate Reductase from the Diatom Thalassiosira pseudonana.

Authors:  N K Amy; R H Garrett
Journal:  Plant Physiol       Date:  1974-10       Impact factor: 8.340
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.