Literature DB >> 16658342

Possible errors in quantitative determination of phenylalanine ammonia-lyase activity by spectrophotometric methods.

A Erez1.   

Abstract

A possible error in spectrophotometric determination of cinnamate, the product of phenylalanine ammonia-lyase activity, using nonpurified protein extracts has been shown.Under optimal conditions for phenylalanine ammonia-lyase activity, with borate buffer and in the presence of alpha-keto acids, phenylpyruvate is produced and its enol tautomer-borate complex formed, strongly absorbs at 290 nanometers.

Entities:  

Year:  1973        PMID： 16658342      PMCID： PMC366277          DOI： 10.1104/pp.51.2.409

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  11 in total

1.  The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare.

Authors:  J KOUKOL; E E CONN
Journal:  J Biol Chem       Date:  1961-10       Impact factor: 5.157

2.  Enzymic catalysis of the keto-enol tautomerization of phenylpyruvic acids.

Authors:  W E KNOX; B M PITT
Journal:  J Biol Chem       Date:  1957-04       Impact factor: 5.157

3.  Effects of Abscisin II on Phenylalanine Ammonia-Lyase Activity in Excised Bean Axes.

Authors:  D C Walton; E Sondheimer
Journal:  Plant Physiol       Date:  1968-03       Impact factor: 8.340

4.  Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings: A Contribution to Eliminate Some Misconceptions.

Authors:  P Schopfer; H Mohr
Journal:  Plant Physiol       Date:  1972-01       Impact factor: 8.340

5.  Induction of Phenylalanine Deaminase by Light and its Relation to Chlorogenic Acid Synthesis in Potato Tuber Tissue.

Authors:  M Zucker
Journal:  Plant Physiol       Date:  1965-09       Impact factor: 8.340

6.  A ssay of L-phenylalaine:2-oxoglutarate aminotransferase by determination of phenyl pyruvate.

Authors:  R Scandurra; C Cannella
Journal:  Anal Biochem       Date:  1969-02       Impact factor: 3.365

7.  Induction of phenylalanine ammonia-lyase in Xanthium leaf disks. Photosynthetic requirement and effect of daylength.

Authors:  M Zucker
Journal:  Plant Physiol       Date:  1969-06       Impact factor: 8.340

8.  Phytochrome-mediated induction of enzyme synthesis in mustard seedlings(Sinapis alba L.).

Authors:  F Durst; H Mohr
Journal:  Naturwissenschaften       Date:  1966-10

9.  Sequential Induction of Phenylalanine Ammonia-lyase and a Lyase-inactivating System in Potato Tuber Disks.

Authors:  M Zucker
Journal:  Plant Physiol       Date:  1968-03       Impact factor: 8.340

10.  Red and far red effects on phenylalanine ammonia-lyase in raphanus and sinapis seedlings do not correlate with phytochrome spectrophotometry.

Authors:  E Bellini; W S Hillman
Journal:  Plant Physiol       Date:  1971-05       Impact factor: 8.340
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  2 in total

1.  Distribution of phenylalanine transaminase and phenylalanine ammonia-lyase activities in etiolated and light irradiated radish seedlings (Raphanus sativus L.).

Authors:  F Tomè; L Campedelli; E Bellini
Journal:  Experientia       Date:  1975-10-15

2.  The Suitability of a Quantitative Spectrophotometric Assay for Phenylalanine Ammonia-lyase Activity in Barley, Buckwheat, and Pea Seedlings.

Authors:  J A Saunders; J W McClure
Journal:  Plant Physiol       Date:  1974-09       Impact factor: 8.340
  2 in total

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