Literature DB >> 16657599

Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation.

A Vasconcelos1, M Pollack, L R Mendiola, H P Hoffmann, D H Brown, C A Price.   

Abstract

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

Entities:  

Year:  1971        PMID： 16657599      PMCID： PMC365845          DOI： 10.1104/pp.47.2.217

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  16 in total

1.  THE ISOLATION OF STRUCTURALLY INTACT CHLOROPLASTS.

Authors:  R M LEECH
Journal:  Biochim Biophys Acta       Date:  1964-05-25

2.  THE PROTEIN-SYNTHESIZING SYSTEMS FROM THE CYTOPLASM AND THE CHLOROPLASTS OF EUGLENA GRACILIS.

Authors:  J M EISENSTADT; G BRAWERMAN
Journal:  J Mol Biol       Date:  1964-12       Impact factor: 5.469

3.  A MEMBRANE METHOD FOR DETERMINATION OF TOTAL PROTEIN IN DILUTE ALGAL SUSPENSIONS.

Authors:  C A PRICE
Journal:  Anal Biochem       Date:  1965-08       Impact factor: 3.365

4.  A comment on the spectrophotometric determination of chlorophyll.

Authors:  J BRUINSMA
Journal:  Biochim Biophys Acta       Date:  1961-09-30

5.  Euglena gracilis, A Test Organism for Study of Zinc.

Authors:  C A Price; B L Vallee
Journal:  Plant Physiol       Date:  1962-05       Impact factor: 8.340

6.  Characterization of macromolecules by constant velocity sedimentation.

Authors:  H Noll
Journal:  Nature       Date:  1967-07-22       Impact factor: 49.962

7.  Sedimentation rates of plastids in an analytical zonal rotor.

Authors:  C A Price; A P Hirvonen
Journal:  Biochim Biophys Acta       Date:  1967-11-28

8.  Early effects of illumination on the activity of some photosynthetic enzymes.

Authors:  S Chen; D McMahon; L Bogorad
Journal:  Plant Physiol       Date:  1967-01       Impact factor: 8.340

9.  A study of fixation for electron microscopy.

Authors:  G E PALADE
Journal:  J Exp Med       Date:  1952-03       Impact factor: 14.307

10.  The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors:  E S REYNOLDS
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539
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  14 in total

1.  Structure and expression of the Euglena gracilis nuclear gene coding for the translation elongation factor EF-1 alpha.

Authors:  P E Montandon; E Stutz
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971

2.  Studies on the isolation and purification of chloroplasts from Euglena gracilis.

Authors:  J F Preston; F Parenti; J M Eisenstadt
Journal:  Planta       Date:  1972-12       Impact factor: 4.116

3.  Preparation of chloroplasts from euglena highly active in protein synthesis.

Authors:  W Ortiz; E M Reardon; C A Price
Journal:  Plant Physiol       Date:  1980-08       Impact factor: 8.340

4.  Euglena gracilis chloroplast DNA: analysis of a 1.6 kb intron of the psb C gene containing an open reading frame of 458 codons.

Authors:  P E Montandon; A Vasserot; E Stutz
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886

5.  Isolation and characterization of cytoplasmic and chloroplastic ribosomes and their ribosomal RNAs from the diatom Cylindrotheca fusiformis.

Authors:  T W Okita; B E Volcani
Journal:  Arch Microbiol       Date:  1977-01-11       Impact factor: 2.552

6.  Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA.

Authors:  P E Montandon; E Stutz
Journal:  Nucleic Acids Res       Date:  1983-09-10       Impact factor: 16.971

7.  The chloroplast and cytoplasmic ribosomes of euglena: I. Stability of chloroplast ribosomes prepared by an improved procedure.

Authors:  S D Schwartzbach; G Freyssinet; J A Schiff
Journal:  Plant Physiol       Date:  1974-04       Impact factor: 8.340

8.  Photosynthetic activity of spinach chloroplasts after isopycnic centrifugation in gradients of silica.

Authors:  J J Morgenthaler; C A Price
Journal:  Plant Physiol       Date:  1974-10       Impact factor: 8.340

9.  Isolation of Intact Chloroplasts of Euglena gracilis by Isopycnic Sedimentation in Gradients of Silica.

Authors:  J L Salisbury; A C Vasconcelos; G L Floyd
Journal:  Plant Physiol       Date:  1975-09       Impact factor: 8.340

10.  The sites of transcription and translation for Euglena chloroplastic aminoacyl-tRNA synthetases.

Authors:  L I Hecker; J Egan; R J Reynolds; C E Nix; J A Schiff; W E Barnett
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205
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