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Literature DB >> 16657435

A simplified purification and some properties of ribulose 1,5-diphosphate carboxylase from barley.

G E Kleinkopf¹, R C Huffaker, A Matheson.

Abstract

A rapid procedure was developed for purifying ribulose 1,5-diphosphate carboxylase from barley leaves. After (NH(4))(2)SO(4) fractionation, the unique sedimentation properties of the enzyme were exploited to effect a single step purification to 90% homogeneity. High speed centrifugation pelleted the enzyme with complete recovery of activity. Residual impurities were then removed by diethylaminoethyl cellulose chromatography and density gradient centrifugation. The purified protein exhibited size heterogeneity due to polymerization. The polymerization products were enzymatically active aggregates of ribulose 1,5-diphosphate carboxylase and were precipitated by an antibody specific for the enzyme.

Entities: Chemical Species

Year: 1970 PMID： 16657435 PMCID： PMC396563 DOI： 10.1104/pp.46.2.204

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

13 in total

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

2. AN IMPROVED METHOD FOR THE ISOLATION OF CARBOXYDISMUTASE. PROBABLE IDENTITY WITH FRACTION I PROTEIN AND THE PROTEIN MOIETY OF PROTOCHLOROPHYLL HOLOCHROME.

Authors: P W TROWN
Journal: Biochemistry Date: 1965-05 Impact factor: 3.162

3. Studies on a protochlorophyll-protein complex. I. Purification and molecular-weight determination.

Authors: N K BOARDMAN
Journal: Biochim Biophys Acta Date: 1962-07-30

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Characterization of ribulose diphosphate carboxylase and phosphoribulokinase from Thiobacillus thioparus and Thiobacillus neapolitanus.

Authors: R D MacElroy; E J Johnson; M K Johnson
Journal: Arch Biochem Biophys Date: 1968-09-20 Impact factor: 4.013

6. Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

Authors: J L Hedrick; A J Smith
Journal: Arch Biochem Biophys Date: 1968-07 Impact factor: 4.013

7. Photosynthesis in Rhodospirillum rubrum. IV. Isolation and characterization of ribulose 1,5-diphosphate carboxylase.

Authors: L E Anderson; R C Fuller
Journal: J Biol Chem Date: 1969-06-25 Impact factor: 5.157

8. Spinach ribulose diphosphate carboxylase. I. Purification and properties of the enzyme.

Authors: J M Paulsen; M D Lane
Journal: Biochemistry Date: 1966-07 Impact factor: 3.162

9. Properties of ribulose-1,5-diphosphate carboxylase (carboxydismutase) from Chinese cabbage and photosynthetic microorganisms.

Authors: F J Kieras; R Haselkorn
Journal: Plant Physiol Date: 1968-08 Impact factor: 8.340

4. An Explanation for the Difference in Photosynthetic Capabilities of Healthy and Beet Yellows Virus-infected Sugar Beets (Beta vulgaris L.).

Authors: A E Hall; R S Loomis
Journal: Plant Physiol Date: 1972-11 Impact factor: 8.340

5. Loss of Ribulose 1,5-Diphosphate Carboxylase and Increase in Proteolytic Activity during Senescence of Detached Primary Barley Leaves.

Authors: L W Peterson; R C Huffaker
Journal: Plant Physiol Date: 1975-06 Impact factor: 8.340

5 in total