Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Gas-Liquid Chromatographic Isolation of Cytokinins from Natural Sources: 6-(3-Methyl-2-butenylamino) purine from Agrobacterium Tumefaciens.

Literature DB >> 16657339

Gas-Liquid Chromatographic Isolation of Cytokinins from Natural Sources: 6-(3-Methyl-2-butenylamino) purine from Agrobacterium Tumefaciens.

C D Upper¹, J P Helgeson, J D Kemp, C J Schmidt.

Abstract

Gas-liquid chromatographic retention times for 20 purines or purine nucleosides, 14 of which are highly active cytokinins, are reported. With one exception, all of the naturally occurring cytokinins are separated.Ethyl acetate extraction of yeast transfer RNA hydrolysates and of culture filtrates of Agrobacterium tumefaciens gave sufficient concentration of the naturally occurring cytokinins for immediate gas-liquid chromatography. This procedure permitted the detection of 6-(3-methyl-2-butenylamino)-9-beta, d-ribofuranosylpurine in yeast transfer RNA extracts. An active cytokinin was isolated from A. tumefaciens culture filtrates and was tentatively identified as 6-(3-methyl-2-butenylamino)purine.

Entities: Chemical Species

Year: 1970 PMID： 16657339 PMCID： PMC396456 DOI： 10.1104/pp.45.5.543

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

15 in total

1. Control of logarithmic growth rates of tobacco callus tissue by cytokinins.

Authors: J P Helgeson; S M Krueger; C D Upper
Journal: Plant Physiol Date: 1969-02 Impact factor: 8.340

2. Isolation of cytokinins from Corynebacterium fascians.

Authors: D Klämbt; G Thies; F Skoog
Journal: Proc Natl Acad Sci U S A Date: 1966-07 Impact factor: 11.205

3. Cytokinins: identification of compounds isolated from Corynebacterium fascians.

Authors: J P Helgeson; N J Leonard
Journal: Proc Natl Acad Sci U S A Date: 1966-07 Impact factor: 11.205

Review 4. Hormonal regulation in higher plants.

Authors: A W Galston; P J Davies
Journal: Science Date: 1969-03-21 Impact factor: 47.728

5. Gas chromatography of cytokinins.

Authors: B H Most; J C Williams; K J Parker
Journal: J Chromatogr Date: 1968-11-05

6. Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotides, nucleosides, and bases.

Authors: J A McCloskey; A M Lawson; K Tsuboyama; P M Krueger; R N Stillwell
Journal: J Am Chem Soc Date: 1968-07-17 Impact factor: 15.419

7. Gas-liquid chromatography of trimethylsilyl derivatives of abscisic Acid and other plant hormones.

Authors: L A Davis; D E Heinz; F T Addicott
Journal: Plant Physiol Date: 1968-09 Impact factor: 8.340

8. A new silylation reagent for amino acids bis(trimethylsilyl)trifluoroacetamide (BSTFA).

Authors: D L Stalling; C W Gehrke; R W Zumwalt
Journal: Biochem Biophys Res Commun Date: 1968-05-23 Impact factor: 3.575

9. Gas chromatographic separation of ribonucleotides by means of trimethylsilyl (TMS) derivatives.

Authors: T Hashizume; Y Sasaki
Journal: Anal Biochem Date: 1966-04 Impact factor: 3.365

10. Synthesis of the "minor nucleotide" N6-(gamma, gamma-dimethylallyl)adenosine 5'-phosphate and relative rates of rearrangement of 1-to N6-dimethylallyl compounds for base, nucleoside, and nucleotide.

Authors: W A Grimm; N J Leonard
Journal: Biochemistry Date: 1967-12 Impact factor: 3.162

14 in total

1. Identification of plant hormones from cotton ovules.

Authors: W W Shindy; O E Smith
Journal: Plant Physiol Date: 1975-03 Impact factor: 8.340

2. A simple, inexpensive, high pressure liquid chromatographic method for separating cytokinins in plant extracts.

Authors: T H Thomas; J E Carroll; F M Isenberg; A Pendergrass; L Howell
Journal: Plant Physiol Date: 1975-09 Impact factor: 8.340

3. Purification and identification of a cytokinin from moss callus cells.

Authors: P Beutelmann; L Bauer
Journal: Planta Date: 1977-01 Impact factor: 4.116

4. Phenotypic and molecular characterisation of efficient nitrogen-fixing Azotobacter strains from rice fields for crop improvement.

Authors: Ranjan K Sahoo; Mohammad W Ansari; Tushar K Dangar; Santanu Mohanty; Narendra Tuteja
Journal: Protoplasma Date: 2013-09-05 Impact factor: 3.356

5. Cytokinins in Corynebacterium fascians Cultures: Isolation and Identification of 6-(4-Hydroxy-3-methyl-cis-2-butenylamino)-2-methylthiopurine.

Authors: D J Armstrong; E Scarbrough; F Skoog
Journal: Plant Physiol Date: 1976-12 Impact factor: 8.340

10. Evaluation of selective detectors for the rapid and sensitive gas chromatographic assay of cytokinins, and application to the analysis of cytokinins in plant extracts.

Authors: R J Whenham
Journal: Planta Date: 1983-05 Impact factor: 4.116