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Literature DB >> 16657106

Nucleoside Diphosphate-sugar 4-Epimerases I. Uridine Diphosphate Glucose 4-Epimerase of Wheat Germ.

Abstract

Uridine diphosphate (UDP)-glucose 4-epimerase (EC 5.1.3.2) has been purified over 1000-fold from extracts of wheat germ by MnCl(2) treatment, (NH(4))(2)SO(4) fractionation, Sephadex column chromatography, and adsorption onto and elution from calcium phosphate gel. The enzyme has a pH optimum of 9.0. Km values are 0.1 mm for UDP-d-galactose and 0.2 mm for UDP-d-glucose. NAD is required for activity; K(a) = 0.04 mm. NADH is an inhibitor strictly competitive with NAD; K(i) = 2 mum. Wheat germ also contains UDP-l-arabinose 4-epimerase (EC 5.1.3.5) and thymidine diphosphate (TDP)-glucose 4-epimerase which are distinct from UDP-glucose 4-epimerase.

Entities: Chemical Disease Species

Year: 1969 PMID： 16657106 PMCID： PMC396131 DOI： 10.1104/pp.44.4.599

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

12 in total

1. THE ENZYMES OF THE GALACTOSE OPERON IN ESCHERICHIA COLI. I. PURIFICATION AND CHARACTERIZATION OF URIDINE DIPHOSPHOGALACTOSE 4-EPIMERASE.

Authors: D B WILSON; D S HOGNESS
Journal: J Biol Chem Date: 1964-08 Impact factor: 5.157

2. The 4-epimerization and decarboxylation of uridine diphosphate D-glucuronic acid by extracts from Phaseolus aureus seedlings.

Authors: D S FEINGOLD; E F NEUFELD; W Z HASSID
Journal: J Biol Chem Date: 1960-04 Impact factor: 5.157

3. Formation and interconversion of sugar nucleotides by plant extracts.

Authors: E F NEUFELD; V GINSBURG; E W PUTMAN; D FANSHIER; W Z HASSID
Journal: Arch Biochem Biophys Date: 1957-07 Impact factor: 4.013

4. The enzymic interconversion of uridine diphosphogalactose and uridine diphosphoglucose.

Authors: E S MAXWELL
Journal: J Biol Chem Date: 1957-11 Impact factor: 5.157

5. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

6. The enzymatic transformation of uridine diphosphate glucose into a galactose derivative.

Authors: L F LELOIR
Journal: Arch Biochem Biophys Date: 1951-09 Impact factor: 4.013

7. The mechanism of action of UDPG dehydrogenase.

Authors: J Zalitis; D S Feingold
Journal: Biochem Biophys Res Commun Date: 1968-06-10 Impact factor: 3.575

8. Metabolic inhibition of mammalian uridine diphosphate galactose 4-epimerase in cell cultures and in tumor cells.

Authors: E A Robinson; H M Kalckar; H Troedsson; K Sanford
Journal: J Biol Chem Date: 1966-06-25 Impact factor: 5.157

9. Biosynthesis of uridine diphosphate D-xylose. II. Uridine diphosphate D-glucuronate carboxy-lyase of Cryptococcus laurentii.

Authors: H Ankel; D S Feingold
Journal: Biochemistry Date: 1966-01 Impact factor: 3.162

10. Epimerization of UDP-glucose and UDP-xylose in Escherichia coli: evidence for a single enzyme.

Authors: H Ankel; U S Maitra
Journal: Biochem Biophys Res Commun Date: 1968-08-13 Impact factor: 3.575

5 in total

1. Changes in enzymic activities of nucleoside diphosphate sugar interconversions during differentiation of cambium to xylem in sycamore and poplar.

Authors: G Dalessandro; D H Northcote
Journal: Biochem J Date: 1977-02-15 Impact factor: 3.857

2. Possible control sites of polysaccharide synthesis during cell growth and wall expansion of pea seedlings (Pisum sativum L.).

Authors: G Dalessandro; D H Northcote
Journal: Planta Date: 1977-01 Impact factor: 4.116