| Literature DB >> 16653227 |
M Hassidim1, R Schwarz, J Lieman-Hurwitz, E Marco, M Ronen-Tarazi, A Kaplan.
Abstract
The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal.Entities:
Year: 1992 PMID: 16653227 PMCID: PMC1075894 DOI: 10.1104/pp.100.4.1982
Source DB: PubMed Journal: Plant Physiol ISSN: 0032-0889 Impact factor: 8.340