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Literature DB >> 16653165

Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods.

H Carvalho¹, S Pereira, C Sunkel, R Salema.

Abstract

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.

Entities: Gene Species

Year: 1992 PMID： 16653165 PMCID： PMC1075827 DOI： 10.1104/pp.100.3.1591

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

6 in total

1. Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase.

Authors: J W Edwards; E L Walker; G M Coruzzi
Journal: Proc Natl Acad Sci U S A Date: 1990-05 Impact factor: 11.205

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Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

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Authors: S V Tingey; G M Coruzzi
Journal: Plant Physiol Date: 1987-06 Impact factor: 8.340

4. Multiple Subunit Composition of Chloroplastic Glutamine Synthetase of Nicotiana tabacum L.

Authors: B Hirel; C Weatherley; C Cretin; C Bergounioux; P Gadal
Journal: Plant Physiol Date: 1984-02 Impact factor: 8.340

5. Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf.

Authors: S F McNally; B Hirel; P Gadal; A F Mann; G R Stewart
Journal: Plant Physiol Date: 1983-05 Impact factor: 8.340

6. Glutamine synthetase of Phaseolus vulgaris L.: organ-specific expression of a multigene family.

Authors: J V Cullimore; C Gebhardt; R Saarelainen; B J Miflin; K B Idler; R F Barker
Journal: J Mol Appl Genet Date: 1984

6 in total

20 in total

1. Biochemical and molecular characterization of transgenic Lotus japonicus plants constitutively over-expressing a cytosolic glutamine synthetase gene.

Authors: Jose Luis Ortega; Stephen J Temple; Suman Bagga; Soumitra Ghoshroy; Champa Sengupta-Gopalan
Journal: Planta Date: 2004-06-10 Impact factor: 4.116

2. Primary N-assimilation into Amino Acids in Arabidopsis.

Authors: Gloria M Coruzzi
Journal: Arabidopsis Book Date: 2003-09-30

Review 3. Use of Arabidopsis mutants and genes to study amide amino acid biosynthesis.

Authors: H M Lam; K Coschigano; C Schultz; R Melo-Oliveira; G Tjaden; I Oliveira; N Ngai; M H Hsieh; G Coruzzi
Journal: Plant Cell Date: 1995-07 Impact factor: 11.277

4. Cellular expression and regulation of the Medicago truncatula cytosolic glutamine synthetase genes in root nodules.

Authors: H Carvalho; N Lescure; F de Billy; M Chabaud; L Lima; R Salema; J Cullimore
Journal: Plant Mol Biol Date: 2000-03 Impact factor: 4.076

5. Biphasic and differential expression of cytosolic glutamine synthetase genes of radish during seed germination and senescence of cotyledons.

Authors: A Watanabe; K Hamada; H Yokoi; A Watanabe
Journal: Plant Mol Biol Date: 1994-12 Impact factor: 4.076

6. Occurrence of Only One Form of Glutamine Synthetase in the Green Alga Monoraphidium braunii.

Authors: J. M. Garcia-Fernandez; A. Lopez-Ruiz; F. Toribio; J. M. Roldan; J. Diez
Journal: Plant Physiol Date: 1994-02 Impact factor: 8.340

7. The Effect of Nitrogen Nutrition on the Cellular Localization of Glutamine Synthetase Isoforms in Barley Roots.

Authors: L. J. Peat; A. K. Tobin
Journal: Plant Physiol Date: 1996-08 Impact factor: 8.340

8. Immunolocalization of a Unique Form of Maize Kernel Glutamine Synthetase Using a Monoclonal Antibody.

Authors: M. J. Muhitch; F. C. Felker; E. W. Taliercio; P. S. Chourey
Journal: Plant Physiol Date: 1995-03 Impact factor: 8.340

9. Ethanolic dehydration and its effect on membrane-bound enzymes of Chlorogloeopsis fritschii and Chlorella pyrenoidosa.

Authors: A K Sallal; N A Nimer
Journal: World J Microbiol Biotechnol Date: 1994-03 Impact factor: 3.312

10. Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids.

Authors: Paula M Melo; Lígia M Lima; Isabel M Santos; Helena G Carvalho; Julie V Cullimore
Journal: Plant Physiol Date: 2003-05 Impact factor: 8.340