Literature DB >> 1664855

Collagenase as an agent for dissolving the zona pellucida of hamster and mouse oocytes.

M Zuccotti1, U A Urch, R Yanagimachi.   

Abstract

Crude preparations of collagenase, which have been used commonly for tissue dissociation, contain proteases that dissolve zonae pellucidae of hamster and mouse oocytes without reducing the ability of the oolemma to fuse with spermatozoa. This gentle proteolytic removal of zona is particularly useful for the study of sperm-oocyte fusion in mice, as trypsin, chymotrypsin and pronase damage the mouse oolemma.

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Year:  1991        PMID： 1664855     DOI： 10.1530/jrf.0.0930515

Source DB:  PubMed          Journal:  J Reprod Fertil        ISSN： 0022-4251


  3 in total

1.  One-step collagenase method for zona pellucida removal in unfertilized eggs: easy and gentle method for large-scale preparation.

Authors:  Kenji Yamatoya; Chizuru Ito; Motoyuki Araki; Ryoji Furuse; Kiyotaka Toshimori
Journal:  Reprod Med Biol       Date:  2011-02-08

2.  Evaluation of zona pellucida function for sperm penetration during in vitro fertilization in pigs.

Authors:  Fuminori Tanihara; Michiko Nakai; Hiroyuki Kaneko; Junko Noguchi; Takeshige Otoi; Kazuhiro Kikuchi
Journal:  J Reprod Dev       Date:  2013-05-11       Impact factor: 2.214

3.  Prolonged exposure to hyaluronidase decreases the fertilization and development rates of fresh and cryopreserved mouse oocytes.

Authors:  Yuta Ishizuka; Toru Takeo; Satohiro Nakao; Hidetaka Yoshimoto; Yumiko Hirose; Yuki Sakai; Yuka Horikoshi; Shiori Takeuji; Shuuji Tsuchiyama; Naomi Nakagata
Journal:  J Reprod Dev       Date:  2014-09-12       Impact factor: 2.214
  3 in total

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