OBJECTIVE: The aim of this study was to develop a mouse model of chronic eosinophilic rhinosinusitis. STUDY DESIGN: Mice were sensitized to Aspergillis fumigatus (Af) extract by intraperitoneal injection. The animals subsequently received nasal challenges with Af extract 3 times per week for 12 weeks. Sinonasal complexes were studied histologically by the study otolaryngologists and pathologists to characterize the inflammatory response. SETTING: Animal care facility at an academic institution. RESULTS: A chronic eosinophilic inflammatory response was evoked in all study animals. Statistical analysis was performed for inflammation, secretory cell hyperplasia, mast cells, and eosinophils. There were very significant differences (P<0.0005) between control and study mice in all categories. CONCLUSION: Prolonged nasal challenge of Af extract creates an inflammatory response in murine nasal mucosa that mimics human chronic eosinophilic rhinosinusitis. SIGNIFICANCE: A murine model for chronic rhinosinusitis is reported that may facilitate future investigations into disease pathophysiology. EBM RATING: B-2.
OBJECTIVE: The aim of this study was to develop a mouse model of chronic eosinophilic rhinosinusitis. STUDY DESIGN:Mice were sensitized to Aspergillis fumigatus (Af) extract by intraperitoneal injection. The animals subsequently received nasal challenges with Af extract 3 times per week for 12 weeks. Sinonasal complexes were studied histologically by the study otolaryngologists and pathologists to characterize the inflammatory response. SETTING: Animal care facility at an academic institution. RESULTS: A chronic eosinophilic inflammatory response was evoked in all study animals. Statistical analysis was performed for inflammation, secretory cell hyperplasia, mast cells, and eosinophils. There were very significant differences (P<0.0005) between control and study mice in all categories. CONCLUSION: Prolonged nasal challenge of Af extract creates an inflammatory response in murine nasal mucosa that mimics human chronic eosinophilic rhinosinusitis. SIGNIFICANCE: A murine model for chronic rhinosinusitis is reported that may facilitate future investigations into disease pathophysiology. EBM RATING: B-2.
Authors: Joy Hsu; Pedro C Avila; Robert C Kern; M Geoffrey Hayes; Robert P Schleimer; Jayant M Pinto Journal: J Allergy Clin Immunol Date: 2013-04 Impact factor: 10.793
Authors: Hannah H Walford; Sean J Lund; Rachel E Baum; Andrew A White; Christopher M Bergeron; Jacob Husseman; Kelly J Bethel; David R Scott; Naseem Khorram; Marina Miller; David H Broide; Taylor A Doherty Journal: Clin Immunol Date: 2014-09-16 Impact factor: 3.969
Authors: Heng Wang; Xiao-Bo Long; Ping-Ping Cao; Nan Wang; Yang Liu; Yong-Hua Cui; Shau-Ku Huang; Zheng Liu Journal: Am J Respir Crit Care Med Date: 2010-01-21 Impact factor: 21.405