| Literature DB >> 16647041 |
Chunlei Li1, Wenjuan Duan, Fuyu Yang, Xujia Zhang.
Abstract
It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here, we describe the isolation and characterization of microdomains from sarcoplasmic reticulum (SR) membranes. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol have a low buoyant density. Immunofluorescence microscopy of SR membranes shows the presence of caveolin-3 in the SR, known as a marker protein of caveolae at plasma membrane. We also demonstrated that significant amount of SERCAs together with caveolin-3 associates with SR-DRMs and are fully functional. Depletion of cholesterol caused the disruption of SR-DRMs.Entities:
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Year: 2006 PMID: 16647041 DOI: 10.1016/j.bbrc.2006.04.024
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN: 0006-291X Impact factor: 3.575