BACKGROUND/AIMS: Although mouse liver contains a large population of B cells, little is known about how hepatic B cells respond to bacterial lipopolysaccharide (LPS). METHODS: The cytokine and IgM productions of hepatic B cells were compared with those of splenic B cells. The effect of LPS-treated hepatic B cells on IFN-gamma production from co-cultured NK1.1+ cells was also examined by irradiation and transwell experiments. RESULTS: Hepatic B cells stimulated with LPS produced substantial amounts of IFN-gamma and IL-12 but a small amount of IgM, while splenic B cells did not produce any of these cytokines but produced a large amount of IgM. The hepatic B cells expressed surface markers similar to those on spleen B cells but expressed more C-X-C chemokine receptor 3 than spleen B cells. Notably, depletion of B220+ cells from liver MNCs (but not from spleen MNCs) greatly decreased LPS-induced IFN-gamma production. Furthermore, LPS-treated hepatic B cells stimulated liver NK1.1+ cells to produce a remarkable amount of IFN-gamma, not only through their soluble factors but also through direct cell-cell contact. CONCLUSIONS: Liver B cells may play an important role in the defense against gram-negative bacterial infections by inducing IFN-gamma production from liver NK cells.
BACKGROUND/AIMS: Although mouse liver contains a large population of B cells, little is known about how hepatic B cells respond to bacterial lipopolysaccharide (LPS). METHODS: The cytokine and IgM productions of hepatic B cells were compared with those of splenic B cells. The effect of LPS-treated hepatic B cells on IFN-gamma production from co-cultured NK1.1+ cells was also examined by irradiation and transwell experiments. RESULTS: Hepatic B cells stimulated with LPS produced substantial amounts of IFN-gamma and IL-12 but a small amount of IgM, while splenic B cells did not produce any of these cytokines but produced a large amount of IgM. The hepatic B cells expressed surface markers similar to those on spleen B cells but expressed more C-X-C chemokine receptor 3 than spleen B cells. Notably, depletion of B220+ cells from liver MNCs (but not from spleen MNCs) greatly decreased LPS-induced IFN-gamma production. Furthermore, LPS-treated hepatic B cells stimulated liver NK1.1+ cells to produce a remarkable amount of IFN-gamma, not only through their soluble factors but also through direct cell-cell contact. CONCLUSIONS: Liver B cells may play an important role in the defense against gram-negative bacterial infections by inducing IFN-gamma production from liver NK cells.
Authors: Laura Gomez-Santos; Zigmund Luka; Conrad Wagner; Sara Fernandez-Alvarez; Shelly C Lu; Jose M Mato; Maria L Martinez-Chantar; Naiara Beraza Journal: Hepatology Date: 2012-07-06 Impact factor: 17.425
Authors: María P Jiménez de Bagüés; Alba de Martino; Juan F Quintana; Ana Alcaraz; Julián Pardo Journal: Infect Immun Date: 2011-08-08 Impact factor: 3.441
Authors: Natalia F Smirnova; Thomas M Conlon; Carmela Morrone; Peter Dorfmuller; Marc Humbert; Georgios T Stathopoulos; Stephan Umkehrer; Franz Pfeiffer; Ali Ö Yildirim; Oliver Eickelberg Journal: JCI Insight Date: 2019-02-07
Authors: Ashlesh K Murthy; Bharat K R Chaganty; Weidang Li; M Neal Guentzel; James P Chambers; J Seshu; Guangming Zhong; Bernard P Arulanandam Journal: FEMS Immunol Med Microbiol Date: 2009-03