Stephen G Tristram1, Scott Nichols. 1. School of Human Life Sciences, University of Tasmania Launceston, Tasmania 7250, Australia. Stephen.Tristram@utas.edu.au
Abstract
OBJECTIVES: To establish a multiplex PCR to detect and differentiate the beta-lactamase genes of Haemophilus influenzae. METHODS: A collection of H. influenzae isolates with a range of different beta-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study. RESULTS: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region. CONCLUSIONS: The multiplex PCR will be a useful tool for the surveillance of beta-lactamase genes in H. influenzae.
OBJECTIVES: To establish a multiplex PCR to detect and differentiate the beta-lactamase genes of Haemophilus influenzae. METHODS: A collection of H. influenzae isolates with a range of different beta-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study. RESULTS: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region. CONCLUSIONS: The multiplex PCR will be a useful tool for the surveillance of beta-lactamase genes in H. influenzae.
Authors: Adriana Ortega; Jesús Oteo; Maitane Aranzamendi-Zaldumbide; Rosa M Bartolomé; Germán Bou; Emilia Cercenado; M Carmen Conejo; Juan José González-López; Mercedes Marín; Luis Martínez-Martínez; María Merino; Ferran Navarro; Antonio Oliver; Alvaro Pascual; Alba Rivera; Jesús Rodríguez-Baño; Irene Weber; Belén Aracil; José Campos Journal: Antimicrob Agents Chemother Date: 2012-04-09 Impact factor: 5.191
Authors: Ana Raquel Barbosa; Maria Giufrè; Marina Cerquetti; Maria Paula Bajanca-Lavado Journal: J Antimicrob Chemother Date: 2011-01-25 Impact factor: 5.790
Authors: A Aguirre-Quiñonero; I C Pérez Del Molino; C García de la Fuente; M C Sanjuán; J Agüero; L Martínez-Martínez Journal: Eur J Clin Microbiol Infect Dis Date: 2018-05-13 Impact factor: 3.267