Literature DB >> 1664099

Purification of ferredoxin-NADP+ reductase, flavodoxin and ferredoxin from a single batch of the cyanobacterium Anabaena PCC 7119.

J J Pueyo1, C Gómez-Moreno.   

Abstract

Methods are described for the simultaneous isolation of ferredoxin-NADP+ reductase, ferredoxin and flavodoxin from large quantities of the cyanobacterium Anabaena PCC 7119 allowing the use of a single batch of cells. The ultraviolet-visible spectra and the extinction coefficients of ferredoxin-NADP+ reductase and ferredoxin were determined. The purification procedure also yields enriched fractions of phycobiliproteins and cytochrome c553.

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Year:  1991        PMID: 1664099     DOI: 10.1080/10826069108018571

Source DB:  PubMed          Journal:  Prep Biochem        ISSN: 0032-7484


  8 in total

1.  Differential activities of heterocyst ferredoxin, vegetative cell ferredoxin, and flavodoxin as electron carriers in nitrogen fixation and photosynthesis in Anabaena sp.

Authors:  P Razquin; S Schmitz; M L Peleato; M F Fillat; C Gómez-Moreno; H Böhme
Journal:  Photosynth Res       Date:  1995-01       Impact factor: 3.573

2.  Binding thermodynamics of ferredoxin:NADP+ reductase: two different protein substrates and one energetics.

Authors:  Marta Martínez-Júlvez; Milagros Medina; Adrián Velázquez-Campoy
Journal:  Biophys J       Date:  2009-06-17       Impact factor: 4.033

Review 3.  Interaction and electron transfer between ferredoxin-NADP+ oxidoreductase and its partners: structural, functional, and physiological implications.

Authors:  Paula Mulo; Milagros Medina
Journal:  Photosynth Res       Date:  2017-03-30       Impact factor: 3.573

4.  Interaction of Ferredoxin-NADP(+) Reductase with its Substrates: Optimal Interaction for Efficient Electron Transfer.

Authors:  Milagros Medina; Carlos Gómez-Moreno
Journal:  Photosynth Res       Date:  2004-02       Impact factor: 3.573

5.  Purification, cofactor analysis, and site-directed mutagenesis of Synechococcus ferredoxin-nitrate reductase.

Authors:  Luis M Rubio; Enrique Flores; Antonia Herrero
Journal:  Photosynth Res       Date:  2002       Impact factor: 3.573

6.  Kinetics and thermodynamics of the binding of riboflavin, riboflavin 5'-phosphate and riboflavin 3',5'-bisphosphate by apoflavodoxins.

Authors:  J J Pueyo; G P Curley; S G Mayhew
Journal:  Biochem J       Date:  1996-02-01       Impact factor: 3.857

7.  How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls.

Authors:  Guangyu E Chen; Nathan B P Adams; Philip J Jackson; Mark J Dickman; C Neil Hunter
Journal:  Nat Plants       Date:  2021-03-15       Impact factor: 15.793

8.  Changing the tracks: screening for electron transfer proteins to support hydrogen production.

Authors:  Alexander Günzel; Vera Engelbrecht; Thomas Happe
Journal:  J Biol Inorg Chem       Date:  2022-08-29       Impact factor: 3.862

  8 in total

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