Literature DB >> 16637014

Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation.

Marek Sebela1, Tat'ána Stosová, Jan Havlis, Natalie Wielsch, Henrik Thomas, Zbynek Zdráhal, Andrej Shevchenko.   

Abstract

Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.

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Year:  2006        PMID： 16637014     DOI： 10.1002/pmic.200500576

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  9 in total

1.  Purification, crystallization and preliminary crystallographic study of a recombinant plant aminoaldehyde dehydrogenase from Pisum sativum.

Authors:  Martina Tylichová; Pierre Briozzo; David Kopecný; Julien Ferrero; Solange Moréra; Nathalie Joly; Jacques Snégaroff; Marek Sebela
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-01-18

Review 2.  Nanobiocatalysis for protein digestion in proteomic analysis.

Authors:  Jungbae Kim; Byoung Chan Kim; Daniel Lopez-Ferrer; Konstantinos Petritis; Richard D Smith
Journal:  Proteomics       Date:  2010-02       Impact factor: 3.984

3.  Extensive lysine methylation in hyperthermophilic crenarchaea: potential implications for protein stability and recombinant enzymes.

Authors:  Catherine H Botting; Paul Talbot; Sonia Paytubi; Malcolm F White
Journal:  Archaea       Date:  2010-08-05       Impact factor: 3.273

4.  Functional expression of amine oxidase from Aspergillus niger (AO-I) in Saccharomyces cerevisiae.

Authors:  Katerina Kolaríková; Petr Galuszka; Iva Sedlárová; Marek Sebela; Ivo Frébort
Journal:  Mol Biol Rep       Date:  2007-09-27       Impact factor: 2.316

5.  Plant ALDH10 family: identifying critical residues for substrate specificity and trapping a thiohemiacetal intermediate.

Authors:  David Kopečny; Radka Končitíková; Martina Tylichová; Armelle Vigouroux; Hana Moskalíková; Miroslav Soural; Marek Šebela; Solange Moréra
Journal:  J Biol Chem       Date:  2013-02-13       Impact factor: 5.157

6.  Development and characterization of lipidic cochleate containing recombinant factor VIII.

Authors:  Razvan D Miclea; Prashant R Varma; Aaron Peng; Sathy V Balu-Iyer
Journal:  Biochim Biophys Acta       Date:  2007-08-19

7.  Purification of Maize Nucleotide Pyrophosphatase/Phosphodiesterase Casts Doubt on the Existence of Zeatin Cis-Trans Isomerase in Plants.

Authors:  Tomáš Hluska; Marek Šebela; René Lenobel; Ivo Frébort; Petr Galuszka
Journal:  Front Plant Sci       Date:  2017-08-23       Impact factor: 5.753

8.  Intact cell MALDI-TOF mass spectrometric analysis of Chroococcidiopsis cyanobacteria for classification purposes and identification of possible marker proteins.

Authors:  Marek Šebela; Eva Jahodářová; Martin Raus; René Lenobel; Petr Hašler
Journal:  PLoS One       Date:  2018-11-29       Impact factor: 3.240

9.  Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula × Tina.

Authors:  Ondřej Kocáb; Jana Jakšová; Ondřej Novák; Ivan Petřík; René Lenobel; Ivo Chamrád; Andrej Pavlovič
Journal:  J Exp Bot       Date:  2020-06-22       Impact factor: 6.992
  9 in total

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