Literature DB >> 16636918

Semi-anaerobic growth conditions are favoured by some Escherichia coli strains during heterologous expression of some archaeal proteins.

Volkan Demir1, H Benan Dincturk.   

Abstract

Host cell physiology is known to play a crucial role in the expression of foreign genes in heterologous systems. Expression of archaeal genes in anaerobic or semi-anaerobic growth conditions of E. coli has been previously reported to be a means of improving solubility of some proteins. Here, we report that some of the Rosetta strains of E. coli, which harbour the rare tRNA genes for the expression of archaeal genes, favour semi-anaerobic conditions for the expression of putative FMN binding domain of glutamate synthase from Methanocaldococcus jannaschii at low inducer concentrations.

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Year:  2006        PMID： 16636918     DOI： 10.1007/s11033-005-5067-5

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  16 in total

1.  The evolution of glutamate synthase.

Authors:  H B Dincturk; D B Knaff
Journal:  Mol Biol Rep       Date:  2000-09       Impact factor: 2.316

2.  Properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803. Comparison with the Azospirillum brasilense NADPH-dependent enzyme and its isolated alpha subunit.

Authors:  Sergio Ravasio; Laura Dossena; Eugenio Martin-Figueroa; Francisco J Florencio; Andrea Mattevi; Paola Morandi; Bruno Curti; Maria A Vanoni
Journal:  Biochemistry       Date:  2002-06-25       Impact factor: 3.162

3.  Pyruvate formate-lyase-activating enzyme: strictly anaerobic isolation yields active enzyme containing a [3Fe-4S](+) cluster.

Authors:  J B Broderick; T F Henshaw; J Cheek; K Wojtuszewski; S R Smith; M R Trojan; R M McGhan; A Kopf; M Kibbey; W E Broderick
Journal:  Biochem Biophys Res Commun       Date:  2000-03-16       Impact factor: 3.575

Review 4.  Structure--function studies on the iron-sulfur flavoenzyme glutamate synthase: an unexpectedly complex self-regulated enzyme.

Authors:  Maria A Vanoni; Bruno Curti
Journal:  Arch Biochem Biophys       Date:  2005-01-01       Impact factor: 4.013

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 6.  Glutamate synthase: structural, mechanistic and regulatory properties, and role in the amino acid metabolism.

Authors:  Akira Suzuki; David B Knaff
Journal:  Photosynth Res       Date:  2005       Impact factor: 3.573

7.  Existence of two ferredoxin-glutamate synthases in the cyanobacterium Synechocystis sp. PCC 6803. Isolation and insertional inactivation of gltB and gltS genes.

Authors:  F Navarro; S Chávez; P Candau; F J Florencio
Journal:  Plant Mol Biol       Date:  1995-02       Impact factor: 4.076

8.  Whole-genome DNA microarray analysis of a hyperthermophile and an archaeon: Pyrococcus furiosus grown on carbohydrates or peptides.

Authors:  Gerrit J Schut; Scott D Brehm; Susmita Datta; Michael W W Adams
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

9.  Characterization of the flavins and the iron-sulfur centers of glutamate synthase from Azospirillum brasilense by absorption, circular dichroism, and electron paramagnetic resonance spectroscopies.

Authors:  M A Vanoni; D E Edmondson; G Zanetti; B Curti
Journal:  Biochemistry       Date:  1992-05-19       Impact factor: 3.162

10.  Geomicrobiology of deep-sea hydrothermal vents.

Authors:  H W Jannasch; M J Mottl
Journal:  Science       Date:  1985-08-23       Impact factor: 47.728
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