PURPOSE: We studied the age-related changes in graft livers, and their impact on post-transplantation liver function and the growth of young recipient rats. METHODS: Rats aged 11-68 weeks old were studied as controls to assess liver histology, liver function, and body weight. We performed orthotopic liver transplantation using Kamada's cuff technique without arterial reconstruction. Young rats aged 11 weeks were randomized to receive livers from either 11-week-old donors (YD group) or 52-week-old donors (OD group). Recipient rats were killed 0, 8, or 16 weeks after surgery and we assessed the same variables as in the controls. RESULTS: We confirmed an age-related increase in the average size of hepatocytes and their nuclei. These age-related changes persisted and progressed in the graft liver after transplantation. There were no significant differences in the levels of serum transaminases or total bilirubin between the YD and OD groups, but the serum albumin level was significantly lower in the OD group. The YD group grew normally, whereas the OD group recipients lagged significantly in gaining body weight. CONCLUSION: We found that 52-week-old grafts transplanted into 11-week-old recipients resulted in deficient growth and a decline in serum albumin, suggesting that grafted old livers fail to produce enough protein to meet the demands of growth adequately.
PURPOSE: We studied the age-related changes in graft livers, and their impact on post-transplantation liver function and the growth of young recipient rats. METHODS:Rats aged 11-68 weeks old were studied as controls to assess liver histology, liver function, and body weight. We performed orthotopic liver transplantation using Kamada's cuff technique without arterial reconstruction. Young rats aged 11 weeks were randomized to receive livers from either 11-week-old donors (YD group) or 52-week-old donors (OD group). Recipient rats were killed 0, 8, or 16 weeks after surgery and we assessed the same variables as in the controls. RESULTS: We confirmed an age-related increase in the average size of hepatocytes and their nuclei. These age-related changes persisted and progressed in the graft liver after transplantation. There were no significant differences in the levels of serum transaminases or total bilirubin between the YD and OD groups, but the serum albumin level was significantly lower in the OD group. The YD group grew normally, whereas the OD group recipients lagged significantly in gaining body weight. CONCLUSION: We found that 52-week-old grafts transplanted into 11-week-old recipients resulted in deficient growth and a decline in serum albumin, suggesting that grafted old livers fail to produce enough protein to meet the demands of growth adequately.
Authors: S V McDiarmid; J A Gornbein; P J DeSilva; J A Goss; J H Vargas; M G Martín; M E Ament; R W Busuttil Journal: Transplantation Date: 1999-02-15 Impact factor: 4.939
Authors: T Ikegami; T Nishizaki; K Yanaga; M Shimada; K Kishikawa; K Nomoto; H Uchiyama; K Sugimachi Journal: Transplantation Date: 2000-12-27 Impact factor: 4.939
Authors: M Ishikawa; H Kitatani; T Akiyama; Y Shimizu; G A Balderson; R W Shepherd; S V Lynch; T H Ong; R W Strong Journal: Surg Today Date: 1994 Impact factor: 2.549