BACKGROUND: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI) to detect putative breast cancer markers in saliva. METHODS: Salivary specimens were analyzed as either pooled cancer saliva specimens, or individual specimens from healthy women and women diagnosed with carcinoma of the breast. The specimens were applied to a variety of protein chip arrays, washed extensively to remove unbound analytes and analyzed on a SELDI mass spectrometer. RESULTS: The results of this initial study suggest that the WCX protein chip array prepared and washed at pH 3.5 yielded the most promising results. Additionally, the analyses revealed a number of proteins that were higher in intensity among the cancer subjects when compared with controls. These salivary proteins were present at the 18, 113, 170, 228 and 287 km/z ranges using SELDI analyses. CONCLUSIONS: The study suggests that saliva may be useful for high-throughput biomarker discovery.
BACKGROUND: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI) to detect putative breast cancer markers in saliva. METHODS: Salivary specimens were analyzed as either pooled cancer saliva specimens, or individual specimens from healthy women and women diagnosed with carcinoma of the breast. The specimens were applied to a variety of protein chip arrays, washed extensively to remove unbound analytes and analyzed on a SELDI mass spectrometer. RESULTS: The results of this initial study suggest that the WCX protein chip array prepared and washed at pH 3.5 yielded the most promising results. Additionally, the analyses revealed a number of proteins that were higher in intensity among the cancer subjects when compared with controls. These salivary proteins were present at the 18, 113, 170, 228 and 287 km/z ranges using SELDI analyses. CONCLUSIONS: The study suggests that saliva may be useful for high-throughput biomarker discovery.
Authors: Sompop Bencharit; Sandra K Altarawneh; Sarah Schwartz Baxter; Jim Carlson; Gary F Ross; Michael B Border; C Russell Mack; Warren C Byrd; Christopher F Dibble; Silvana Barros; Zvi Loewy; Steven Offenbacher Journal: Mol Biosyst Date: 2012-10-30
Authors: Jesse V Jokerst; Archana Raamanathan; Nicolaos Christodoulides; Pierre N Floriano; Amanda A Pollard; Glennon W Simmons; Jorge Wong; Carole Gage; Wieslaw B Furmaga; Spencer W Redding; John T McDevitt Journal: Biosens Bioelectron Date: 2009-05-27 Impact factor: 10.618
Authors: Fatima Ardito; Donatella Perrone; Roberto Cocchi; Lucio Lo Russo; Alfredo DE Lillo; Giovanni Giannatempo; Lorenzo Lo Muzio Journal: Oncol Lett Date: 2016-02-09 Impact factor: 2.967
Authors: Chandrika J Piyathilake; Denise K Oelschlager; Sreelatha Meleth; Edward E Partridge; William E Grizzle Journal: Cancer Inform Date: 2007-02-27