OBJECTIVE: To determine the prevalent subtypes of feline immunodeficiency virus (FIV) present in the domestic cat population of Australia. METHOD: Blood samples were collected from 41 FIV antibody positive cats from four cities across Australia. Following DNA extraction, polymerase chain reaction (PCR) was performed to amplify the variable V3-V5 region of the envelope (env) gene. Genotypes were assessed by direct sequencing of PCR products and comparison with previously reported FIV sequences. Phylogenetic analysis allowed classification of the Australian sequences into the appropriate subtype. RESULTS: Of the 41 FIV samples, 40 were found to cluster with previously reported subtype A isolates, whilst the remaining sample grouped within subtype B. CONCLUSIONS: Subtype A was found to be the predominant FIV subtype present in Australia, although subtype B was also found. These results broaden our knowledge of the genetic diversity of FIV and the associated implications for preventative, diagnostic and therapeutic approaches.
OBJECTIVE: To determine the prevalent subtypes of feline immunodeficiency virus (FIV) present in the domestic cat population of Australia. METHOD: Blood samples were collected from 41 FIV antibody positive cats from four cities across Australia. Following DNA extraction, polymerase chain reaction (PCR) was performed to amplify the variable V3-V5 region of the envelope (env) gene. Genotypes were assessed by direct sequencing of PCR products and comparison with previously reported FIV sequences. Phylogenetic analysis allowed classification of the Australian sequences into the appropriate subtype. RESULTS: Of the 41 FIV samples, 40 were found to cluster with previously reported subtype A isolates, whilst the remaining sample grouped within subtype B. CONCLUSIONS: Subtype A was found to be the predominant FIV subtype present in Australia, although subtype B was also found. These results broaden our knowledge of the genetic diversity of FIV and the associated implications for preventative, diagnostic and therapeutic approaches.
Authors: Jesse Thompson; Martha MacMillan; Karen Boegler; Charles Wood; John H Elder; Sue VandeWoude Journal: PLoS One Date: 2011-08-26 Impact factor: 3.240
Authors: Paweł M Bęczkowski; Joseph Hughes; Roman Biek; Annette Litster; Brian J Willett; Margaret J Hosie Journal: Retrovirology Date: 2014-09-17 Impact factor: 4.602
Authors: M E Westman; S J Coggins; M van Dorsselaer; J M Norris; R A Squires; M Thompson; R Malik Journal: Aust Vet J Date: 2022-05-16 Impact factor: 1.343
Authors: Julia Frankenfeld; Theres Meili; Marina L Meli; Barbara Riond; A Katrin Helfer-Hungerbuehler; Eva Bönzli; Benita Pineroli; Regina Hofmann-Lehmann Journal: Viruses Date: 2019-07-31 Impact factor: 5.048
Authors: Margaret J Hosie; Diane Addie; Sándor Belák; Corine Boucraut-Baralon; Herman Egberink; Tadeusz Frymus; Tim Gruffydd-Jones; Katrin Hartmann; Albert Lloret; Hans Lutz; Fulvio Marsilio; Maria Grazia Pennisi; Alan D Radford; Etienne Thiry; Uwe Truyen; Marian C Horzinek Journal: J Feline Med Surg Date: 2009-07 Impact factor: 2.015
Authors: Anna Szilasi; Lilla Dénes; Eszter Krikó; Kristin Heenemann; Reinhard Ertl; Míra Mándoki; Thomas W Vahlenkamp; Gyula Balka Journal: JFMS Open Rep Date: 2019-12-10