OBJECTIVES: Tissue concentrations of amphotericin B were determined in autopsy material of patients who had been treated with liposomal amphotericin B or amphotericin B colloidal dispersion (colloidal amphotericin B) for suspected or proven invasive fungal infection. PATIENTS AND METHODS: Amphotericin B tissue levels were measured in liver, spleen, lung, kidney, and myocardial and brain tissue of 20 patients who had been treated with lipid-formulated amphotericin B, before they died from multi-organ failure. Seven patients had been treated with liposomal amphotericin B (AmBisome) and thirteen with colloidal amphotericin B (Amphocil). Tissue samples were obtained during routine autopsy, homogenized and extracted with methanol. Amphotericin B concentrations were measured using HPLC after purification by solid phase extraction. RESULTS: The highest amphotericin B levels were found in liver and spleen, followed by kidney, lung, myocardium and brain. In the lung higher amphotericin B concentrations were found after treatment with amphotericin B colloidal dispersion than after therapy with liposomal amphotericin B. CONCLUSIONS: The choice of lipid formulation may influence amphotericin B penetration into the lung.
OBJECTIVES: Tissue concentrations of amphotericin B were determined in autopsy material of patients who had been treated with liposomal amphotericin B or amphotericin B colloidal dispersion (colloidal amphotericin B) for suspected or proven invasive fungal infection. PATIENTS AND METHODS: Amphotericin B tissue levels were measured in liver, spleen, lung, kidney, and myocardial and brain tissue of 20 patients who had been treated with lipid-formulated amphotericin B, before they died from multi-organ failure. Seven patients had been treated with liposomal amphotericin B (AmBisome) and thirteen with colloidal amphotericin B (Amphocil). Tissue samples were obtained during routine autopsy, homogenized and extracted with methanol. Amphotericin B concentrations were measured using HPLC after purification by solid phase extraction. RESULTS: The highest amphotericin B levels were found in liver and spleen, followed by kidney, lung, myocardium and brain. In the lung higher amphotericin B concentrations were found after treatment with amphotericin B colloidal dispersion than after therapy with liposomal amphotericin B. CONCLUSIONS: The choice of lipid formulation may influence amphotericin B penetration into the lung.
Authors: Valdir S Amato; Felipe F Tuon; Raphael A Camargo; Regina M Souza; Carolina R Santos; Antonio C Nicodemo Journal: Am J Trop Med Hyg Date: 2011-11 Impact factor: 2.345
Authors: Stefan Weiler; David Fiegl; Róisín MacFarland; Eva Stienecke; Rosa Bellmann-Weiler; Stefan Dunzendorfer; Michael Joannidis; Romuald Bellmann Journal: Antimicrob Agents Chemother Date: 2010-11-15 Impact factor: 5.191
Authors: Joseph N Jarvis; Tshepo B Leeme; Mooketsi Molefi; Awilly A Chofle; Gabriella Bidwell; Katlego Tsholo; Nametso Tlhako; Norah Mawoko; Raju K K Patel; Mark W Tenforde; Charles Muthoga; Gregory P Bisson; Jeremiah Kidola; John Changalucha; David Lawrence; Shabbar Jaffar; William Hope; Si le F Molloy; Thomas S Harrison Journal: Clin Infect Dis Date: 2019-01-18 Impact factor: 9.079