Literature DB >> 1662700

Conversion of a single-stranded simian virus 40 (SV40)-based shuttle vector to its double-stranded form does not require the SV40 T antigen in monkey cells.

C Madzak1, A Sarasin.   

Abstract

We have transfected the single-stranded DNA form of the simian virus 40 (SV40)-based shuttle vector pZ189 into CV1P simian cells. Although the strand used did not code for the T antigen, we observed its conversion to a double-stranded DNA form. We deduced that the replication step converting a circular single-stranded DNA to a double-stranded one is independent of the SV40 T antigen in simian cells.

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Year:  1991        PMID： 1662700     DOI： 10.1099/0022-1317-72-12-3091

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  2 in total

1.  Single-stranded shuttle phagemid for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA induces targeted G.C-->T.A transversions in simian kidney cells.

Authors:  M Moriya
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-01       Impact factor: 11.205

2.  Mutagenesis in monkey cells of a vector containing a single d(GPG) cis-diamminedichloroplatinum(II) adduct placed on codon 13 of the human H-ras proto-oncogene.

Authors:  M J Pillaire; A Margot; G Villani; A Sarasin; M Defais; A Gentil
Journal:  Nucleic Acids Res       Date:  1994-07-11       Impact factor: 16.971
  2 in total

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