| Literature DB >> 16624485 |
Gilbert Spizzo1, Guenther Gastl, Peter Obrist, Dominic Fong, Margot Haun, Kurt Grünewald, Walther Parson, Cordula Eichmann, Simone Millinger, Heidi Fiegl, Raimund Margreiter, Albert Amberger.
Abstract
We examined the methylation status of the Ep-CAM promoter region of human breast cancer cell lines and breast cancer tissue using MethyLight technology and bisulfite sequencing. We found the promoter of Ep-CAM-negative breast cancer cell lines Hs 578T to be methylated to a higher degree as compared to positive cell lines MCF-7. Demethylation of cell lines was performed using 5-aza-2'-deoxycytidine. Ep-CAM RNA and protein expression could be partially restored by treating cells with 5-Aza-2'-deoxycytidine. In most primary breast cancer tissue, methylation of the Ep-CAM gene could be detected at a low level and no correlation was found with Ep-CAM protein expression in tumour tissue. Taken together, these data suggest that methylation of the Ep-CAM promoter is not a crucial mechanism for regulation of Ep-CAM expression in breast cancer. Thus, most important regulatory mechanisms have to be supposed in vivo.Entities:
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Year: 2006 PMID： 16624485 DOI： 10.1016/j.canlet.2006.03.002
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679