BACKGROUND: As a link between periodontal infections and an increased risk for vascular disease has been demonstrated, we assessed the ability of the Gram-negative periodontal pathogen Porphyromonas gingivalis to modulate properties of endothelial cells linked to inflammation and proatherogenic pathways. METHODS AND RESULTS: Primary human aortic endothelial cells (HAEC) were infected with either P. gingivalis strain 381 or its non-invasive fimbriae-deficient mutant, DPG3, and incubated with U-937 monocytes, or Jurkat T cells. P. gingivalis-infected HAEC demonstrated significantly increased adhesion of immune cells compared to non-infected cells or those infected with DPG3. Heat-killed bacteria had no effect on mononuclear cell adhesion and P. gingivalis LPS had only a minimal effect. P. gingivalis infection significantly increased HAEC expression of VCAM-1, ICAM-1 and E-selectin, and enhanced production of IL-6, IL-8 and MCP-1. CONCLUSION: These data demonstrate that live invasive P. gingivalis 381 elicits a pro-atherogenic response in HAEC.
BACKGROUND: As a link between periodontal infections and an increased risk for vascular disease has been demonstrated, we assessed the ability of the Gram-negative periodontal pathogen Porphyromonas gingivalis to modulate properties of endothelial cells linked to inflammation and proatherogenic pathways. METHODS AND RESULTS: Primary human aortic endothelial cells (HAEC) were infected with either P. gingivalis strain 381 or its non-invasive fimbriae-deficient mutant, DPG3, and incubated with U-937 monocytes, or Jurkat T cells. P. gingivalis-infected HAEC demonstrated significantly increased adhesion of immune cells compared to non-infected cells or those infected with DPG3. Heat-killed bacteria had no effect on mononuclear cell adhesion and P. gingivalisLPS had only a minimal effect. P. gingivalis infection significantly increased HAEC expression of VCAM-1, ICAM-1 and E-selectin, and enhanced production of IL-6, IL-8 and MCP-1. CONCLUSION: These data demonstrate that live invasive P. gingivalis 381 elicits a pro-atherogenic response in HAEC.
Authors: Panos N Papapanou; Michael H Sedaghatfar; Ryan T Demmer; Dana L Wolf; Jun Yang; Georg A Roth; Romanita Celenti; Paul B Belusko; Evanthia Lalla; Paul Pavlidis Journal: J Clin Periodontol Date: 2007-09 Impact factor: 8.728
Authors: Irina N Baranova; Roger Kurlander; Alexander V Bocharov; Tatyana G Vishnyakova; Zhigang Chen; Alan T Remaley; Gyorgy Csako; Amy P Patterson; Thomas L Eggerman Journal: J Immunol Date: 2008-11-15 Impact factor: 5.422
Authors: Georg A Roth; Klaus Aumayr; Mary Beth Giacona; Panos N Papapanou; Ann Marie Schmidt; Evanthia Lalla Journal: Thromb Res Date: 2008-09-11 Impact factor: 3.944
Authors: Jan H Behle; Michael H Sedaghatfar; Ryan T Demmer; Dana L Wolf; Romanita Celenti; Moritz Kebschull; Paul B Belusko; Miriam Herrera-Abreu; Evanthia Lalla; Panos N Papapanou Journal: J Clin Periodontol Date: 2009-03-11 Impact factor: 8.728