BACKGROUND: The tumor suppressor gene PTEN, mutated in 40-50% of patients with brain tumors, especially those with glioblastomas, maps to chromosome 10q23.3 and encodes a dual-specificity phosphatase. PTEN exerts its effects partly via inhibition of protein tyrosine kinase B (Akt/Protein Kinase B), which is involved in the phosphatidylinositol (PtdIns) 3-kinase (PI3K)-mediated cell-survival pathway. The naturally occurring bioflavonoid Quercetin (Qu) shares structural homology with the commercially available selective PI3K inhibitor, LY 294002 (LY). Here, the effects of Qu on the Akt/PKB pathway were evaluated. MATERIALS AND METHODS: The human breast carcinoma cell lines, HCC1937, with homozygous deletion of the PTEN gene, and T47D, with intact PTEN, were time-treated with Qu or LY and analyzed for activated levels of Akt by measuring phospho-Akt (p-Akt) levels using immunoblotting analysis. To detect p-Akt, the T47D cells were treated with EGF prior to treatment with or without Qu or LY Cell proliferation after 24-h treatment with Qu or LY was quantified by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Treatment with Qu (25 microM) for 0.5, 1 and 3 h completely suppressed constitutively activated Akt/PKB phosphorylation at Ser-473 in HCC1937 cells. Pre-exposing T47D cells to Qu (25 microM) or LY (10 microM) abrogated EGF-induced Akt/PKB phosphorylation at Ser-473. Both Qu (100 microM) and LY (50 microM) treatments for 24 h significantly decreased cell proliferation, as shown by the MTT assay. CONCLUSION: Pharmacologically safe doses of the naturally occurring bioflavonoid Qu inhibit the PI3K-Akt/PKB pathway, in a manner similar to that of the commercially available LY. Overall, our results indicated that Qu inhibited the constitutively activated-Akt/PKB pathway in PTEN-null cancer cells, and suggest that this compound may have therapeutic benefit against tumorigenesis and cancer progression.
BACKGROUND: The tumor suppressor gene PTEN, mutated in 40-50% of patients with brain tumors, especially those with glioblastomas, maps to chromosome 10q23.3 and encodes a dual-specificity phosphatase. PTEN exerts its effects partly via inhibition of protein tyrosine kinase B (Akt/Protein Kinase B), which is involved in the phosphatidylinositol (PtdIns) 3-kinase (PI3K)-mediated cell-survival pathway. The naturally occurring bioflavonoidQuercetin (Qu) shares structural homology with the commercially available selective PI3K inhibitor, LY 294002 (LY). Here, the effects of Qu on the Akt/PKB pathway were evaluated. MATERIALS AND METHODS: The humanbreast carcinoma cell lines, HCC1937, with homozygous deletion of the PTEN gene, and T47D, with intact PTEN, were time-treated with Qu or LY and analyzed for activated levels of Akt by measuring phospho-Akt (p-Akt) levels using immunoblotting analysis. To detect p-Akt, the T47D cells were treated with EGF prior to treatment with or without Qu or LY Cell proliferation after 24-h treatment with Qu or LY was quantified by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Treatment with Qu (25 microM) for 0.5, 1 and 3 h completely suppressed constitutively activated Akt/PKB phosphorylation at Ser-473 in HCC1937 cells. Pre-exposing T47D cells to Qu (25 microM) or LY (10 microM) abrogated EGF-induced Akt/PKB phosphorylation at Ser-473. Both Qu (100 microM) and LY (50 microM) treatments for 24 h significantly decreased cell proliferation, as shown by the MTT assay. CONCLUSION: Pharmacologically safe doses of the naturally occurring bioflavonoidQu inhibit the PI3K-Akt/PKB pathway, in a manner similar to that of the commercially available LY. Overall, our results indicated that Qu inhibited the constitutively activated-Akt/PKB pathway in PTEN-null cancer cells, and suggest that this compound may have therapeutic benefit against tumorigenesis and cancer progression.
Authors: Linette Castillo-Pichardo; Michelle M Martínez-Montemayor; Joel E Martínez; Kristin M Wall; Luis A Cubano; Suranganie Dharmawardhane Journal: Clin Exp Metastasis Date: 2009-03-18 Impact factor: 5.150
Authors: Cynthia A Warren; Kimberly J Paulhill; Laurie A Davidson; Joanne R Lupton; Stella S Taddeo; Mee Young Hong; Raymond J Carroll; Robert S Chapkin; Nancy D Turner Journal: J Nutr Date: 2008-12-03 Impact factor: 4.798