BACKGROUND: The plasminogen activator-plasmin cascade plays a central role in the progression of solid tumors. The type-1 plasminogen activator inhibitor (PAI-1) is the major physiological regulator of plasminogen activation. PAI-1 is suggested to play a crucial role in tumor cell invasion and metastasis of various solid tumors. The aim of this study was to analyze the clinical and prognostic roles of PAI-1 in epithelial ovarian cancer (OC). MATERIALS AND METHODS: Expression analysis was conducted by immunohistochemistry and ELISA. Tissue sections of paraffin-embedded tumor specimens and fresh-frozen tumor samples from patients with benign and malignant ovarian tumors (OT), who had undergone surgical intervention in the Department of Gynaecology and Obstetrics, Charité, Germany, from 02/01 to 06/02, were used. Correlation analysis with conventional clinical factors, univariate and multivariate analyses were performed using SPSS (SPSS Inc., V.11.0). RESULTS: Sixty-five patients (31 primary OC, 20 recurrent OC, 4 low-malignant potential OT, 6 benign OT, 4 normal ovary) were allocated to this trial. The median age was 57 years (range, 34-86) and the median follow-up was 20 months (range, 0-64). The distributions of (FIGO) tumor stage of all primary OC were: I = 16.1%, II = 3.2%, III = 45.2% and IV = 35.5%. PAI-1 was significantly overexpressed in the tumor epithelium of OC in comparison to the ovarian epithelium of benign OT and normal ovary (p < 0.001). The median PAI-1 level was 1.92-fold higher in malignant OT than in benign OT. Statistical analyses showed no significant correlation between the expression of PAI-1 and clinical parameters. The expression of PAI-1 and the PAI-1 level, according to 3 different cut-off values, showed no prognostic impact in univariate analysis. In multivariate analysis, only tumor stage (FIGO) (p = 0.003) and residual tumor (p = 0.009) remained independent prognostic factors for post-operative survival. CONCLUSION: PAI-1 is significantly overexpressed in OC.
BACKGROUND: The plasminogen activator-plasmin cascade plays a central role in the progression of solid tumors. The type-1 plasminogen activator inhibitor (PAI-1) is the major physiological regulator of plasminogen activation. PAI-1 is suggested to play a crucial role in tumor cell invasion and metastasis of various solid tumors. The aim of this study was to analyze the clinical and prognostic roles of PAI-1 in epithelial ovarian cancer (OC). MATERIALS AND METHODS: Expression analysis was conducted by immunohistochemistry and ELISA. Tissue sections of paraffin-embedded tumor specimens and fresh-frozen tumor samples from patients with benign and malignant ovarian tumors (OT), who had undergone surgical intervention in the Department of Gynaecology and Obstetrics, Charité, Germany, from 02/01 to 06/02, were used. Correlation analysis with conventional clinical factors, univariate and multivariate analyses were performed using SPSS (SPSS Inc., V.11.0). RESULTS: Sixty-five patients (31 primary OC, 20 recurrent OC, 4 low-malignant potential OT, 6 benign OT, 4 normal ovary) were allocated to this trial. The median age was 57 years (range, 34-86) and the median follow-up was 20 months (range, 0-64). The distributions of (FIGO) tumor stage of all primary OC were: I = 16.1%, II = 3.2%, III = 45.2% and IV = 35.5%. PAI-1 was significantly overexpressed in the tumor epithelium of OC in comparison to the ovarian epithelium of benign OT and normal ovary (p < 0.001). The median PAI-1 level was 1.92-fold higher in malignant OT than in benign OT. Statistical analyses showed no significant correlation between the expression of PAI-1 and clinical parameters. The expression of PAI-1 and the PAI-1 level, according to 3 different cut-off values, showed no prognostic impact in univariate analysis. In multivariate analysis, only tumor stage (FIGO) (p = 0.003) and residual tumor (p = 0.009) remained independent prognostic factors for post-operative survival. CONCLUSION:PAI-1 is significantly overexpressed in OC.
Authors: Mark S Carey; Roshan Agarwal; Blake Gilks; Kenneth Swenerton; Steve Kalloger; Jennifer Santos; Zhenlin Ju; Yiling Lu; Fan Zhang; Kevin R Coombes; Dianne Miller; David Huntsman; Gordon B Mills; Bryan T Hennessy Journal: Clin Cancer Res Date: 2010-05-11 Impact factor: 12.531
Authors: Tan A Ince; Aurea D Sousa; Michelle A Jones; J Chuck Harrell; Elin S Agoston; Marit Krohn; Laura M Selfors; Wenbin Liu; Ken Chen; Mao Yong; Peter Buchwald; Bin Wang; Katherine S Hale; Evan Cohick; Petra Sergent; Abigail Witt; Zhanna Kozhekbaeva; Sizhen Gao; Agoston T Agoston; Melissa A Merritt; Rosemary Foster; Bo R Rueda; Christopher P Crum; Joan S Brugge; Gordon B Mills Journal: Nat Commun Date: 2015-06-17 Impact factor: 14.919
Authors: Yaakov Bentov; Theodore J Brown; Mohammad R Akbari; Robert Royer; Harvey Risch; Barry Rosen; John McLaughlin; Ping Sun; Shiyu Zhang; Steven A Narod; Robert F Casper Journal: PLoS One Date: 2009-06-15 Impact factor: 3.240