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Literature DB >> 1661745

Detection of rodent coronaviruses in tissues and cell cultures by using polymerase chain reaction.

F R Homberger¹, A L Smith, S W Barthold.

Abstract

A polymerase chain reaction (PCR) method was developed for the detection of rodent coronaviruses in biological material by using reverse transcriptase and two primers which flanked an M gene sequence of 375 bp. PCR detected all of 11 different strains of mouse hepatitis virus (MHV) as well as rat sialodacryoadenitis virus but not bovine coronavirus or human coronavirus strains OC43 and 229E. The M gene sequences of bovine coronavirus and human coronavirus OC43 are homologous to that of MHV, but minor differences exist in the primer regions, preventing annealing of the primers. For detecting MHV-Y in tissue samples, PCR was faster than and at least as sensitive as either of the two bioassays (infant mouse bioassay and mouse antibody production test) currently used for MHV diagnostic purposes.

Entities: Species

Mesh：

Substances：
Antibodies, Viral

Year: 1991 PMID： 1661745 PMCID： PMC270434 DOI： 10.1128/jcm.29.12.2789-2793.1991

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

23 in total

1. Three intergenic regions of coronavirus mouse hepatitis virus strain A59 genome RNA contain a common nucleotide sequence that is homologous to the 3' end of the viral mRNA leader sequence.

Authors: C J Budzilowicz; S P Wilczynski; S R Weiss
Journal: J Virol Date: 1985-03 Impact factor: 5.103

2. Characterization of the virus of sialodacryoadenitis of rats: a member of the coronavirus group.

Authors: P N Bhatt; D H Percy; A M Jonas
Journal: J Infect Dis Date: 1972-08 Impact factor: 5.226

3. Enterotropic mouse hepatitis virus infection in nude mice.

Authors: S W Barthold; A L Smith; M L Povar
Journal: Lab Anim Sci Date: 1985-12

4. Comparison of isolation in cell culture with conventional and modified mouse antibody production tests for detection of murine viruses.

Authors: M de Souza; A L Smith
Journal: J Clin Microbiol Date: 1989-01 Impact factor: 5.948

5. Persistent rat parvovirus infection in individually housed rats.

Authors: R O Jacoby; E A Johnson; F X Paturzo; D J Gaertner; J L Brandsma; A L Smith
Journal: Arch Virol Date: 1991 Impact factor: 2.574

6. Antigenic relationships among proteins of bovine coronavirus, human respiratory coronavirus OC43, and mouse hepatitis coronavirus A59.

Authors: B G Hogue; B King; D A Brian
Journal: J Virol Date: 1984-08 Impact factor: 5.103

7. Coronavirus MHV-JHM: nucleotide sequence of the mRNA that encodes the membrane protein.

Authors: M Pfleiderer; M A Skinner; S G Siddell
Journal: Nucleic Acids Res Date: 1986-08-11 Impact factor: 16.971

8. Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes.

Authors: W Lapps; B G Hogue; D A Brian
Journal: Virology Date: 1987-03 Impact factor: 3.616

9. Response of genetically susceptible and resistant mice to intranasal inoculation with mouse hepatitis virus JHM.

Authors: S W Barthold; A L Smith
Journal: Virus Res Date: 1987-05 Impact factor: 3.303

10. Sequence analysis of the membrane protein gene of human coronavirus 229E.

Authors: P Jouvenne; C D Richardson; S S Schreiber; M M Lai; P J Talbot
Journal: Virology Date: 1990-02 Impact factor: 3.616

11 in total

1. MHC-genotype of progeny influenced by parental infection.

Authors: T Rülicke; M Chapuisat; F R Homberger; E Macas; C Wedekind
Journal: Proc Biol Sci Date: 1998-04-22 Impact factor: 5.349

2. Responses of mice to murine coronavirus immunization.

Authors: A L Smith; M S de Souza; D Finzi; S W Barthold
Journal: Arch Virol Date: 1992 Impact factor: 2.574

3. Population genetics, community of parasites, and resistance to rodenticides in an urban brown rat (Rattus norvegicus) population.

Authors: Amélie Desvars-Larrive; Michel Pascal; Patrick Gasqui; Jean-François Cosson; Etienne Benoît; Virginie Lattard; Laurent Crespin; Olivier Lorvelec; Benoît Pisanu; Alexandre Teynié; Muriel Vayssier-Taussat; Sarah Bonnet; Philippe Marianneau; Sandra Lacôte; Pascale Bourhy; Philippe Berny; Nicole Pavio; Sophie Le Poder; Emmanuelle Gilot-Fromont; Elsa Jourdain; Abdessalem Hammed; Isabelle Fourel; Farid Chikh; Gwenaël Vourc'h
Journal: PLoS One Date: 2017-09-08 Impact factor: 3.240

4. Nucleotide sequence comparison of the membrane protein genes of three enterotropic strains of mouse hepatitis virus.

Authors: F R Homberger
Journal: Virus Res Date: 1994-01 Impact factor: 3.303

5. Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses.

Authors: Ken-Ichi Hanaki; Fumio Ike; Rika Hatakeyama; Norio Hirano
Journal: J Virol Methods Date: 2012-10-30 Impact factor: 2.014

6. Detection of group 2a coronaviruses with emphasis on bovine and wild ruminant strains. Virus isolation and detection of antibody, antigen, and nucleic acid.

Authors: Mustafa Hasoksuz; Anastasia Vlasova; Linda J Saif
Journal: Methods Mol Biol Date: 2008

7. Detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction.

Authors: X Li; F W Scott
Journal: Vet Microbiol Date: 1994-09 Impact factor: 3.293