| Literature DB >> 16616990 |
Yalou Zhang1, Jean-Mathieu Bart, Patrick Giraudoux, Philip Craig, Dominique Vuitton, Hao Wen.
Abstract
The Xinjiang plateau of western China has been shown to have a high prevalence for human cystic echinococcosis (CE) caused by Echinococcus granulosus, and human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. The domestic dog is suspected to be the primary definitive host for the transmission of both E. granulosus and E. multilocularis to humans in this locality. Seventeen of 30 stray dogs from Hejing County of Xinjiang were found positive for E. granulosus post mortem, and one double infection was suspected. Worm samples were collected, dyed by carmine, and observed microscopically. Carmine staining examination clearly revealed the differences in number of proglottids and appearance of uterine branches and lateral genital pore for those two species of Echinococcus. Furthermore, gene target DNA fragments were amplified for formal identification of the two parasite species, based on 12s rRNA mitochondrial gene. The PCR products were purified and sequenced. Compared with NCBI GenBank, the DNA sequences demonstrated 100% identity with E. granulosus (sheep strain, G1 genotype) and E. multilocularis.Entities:
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Year: 2006 PMID： 16616990 DOI： 10.1016/j.vetpar.2006.03.003
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738