Literature DB >> 16616531

Genotyping of Ureaplasma diversum isolates using pulsed-field electrophoresis.

Melissa Buzinhani1, Marcos R Buim, Maurício Yamaguti, Rosângela C Oliveira, Elena Mettifogo, Jorge Timenetsky.   

Abstract

Ureaplasma diversum has been associated with reproductive disorders in cattle and in the present study genotypic variations among U. diversum isolates obtained from the vaginal mucus of healthy cattle and sick animals were analyzed by enzymatic digestion and pulsed-field gel electrophoresis (PFGE). The influence of time and broth volume was important in obtaining sufficient cell sediment and DNA for PFGE. The method presented a high discriminatory power and satisfactory reproducibility for the analysis of detected variations among U. diversum isolates and strains. Different band profiles and wide genotypic heterogeneity were detected but no association between DNA polymorphism and sick or healthy animals could be established.

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Year:  2006        PMID： 16616531     DOI： 10.1016/j.tvjl.2006.02.001

Source DB:  PubMed          Journal:  Vet J        ISSN： 1090-0233            Impact factor:   2.688


  5 in total

1.  Invasion of Ureaplasma diversum in bovine spermatozoids.

Authors:  Melissa Buzinhani; Maurício Yamaguti; Rosângela C Oliveira; Beatriz A Cortez; Lucas Miranda Marques; Gláucia M Machado-Santelli; Mayra Eo Assumpção; Jorge Timenetsky
Journal:  BMC Res Notes       Date:  2011-10-27

2.  Genome Sequence of Ureaplasma diversum Strain ATCC 49782.

Authors:  Lucas M Marques; Ana M S Guimarães; Hellen B Martins; Izadora S Rezende; Maysa S Barbosa; Guilherme B Campos; Naíla C do Nascimento; Andrea P Dos Santos; Aline T Amorim; Verena M Santos; Joanne B Messick; Jorge Timenetsky
Journal:  Genome Announc       Date:  2015-04-16

3.  Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil.

Authors:  Rosângela C Oliveira; Maurício Yamaguti; Lucas M Marques; Melissa Buzinhani; Huber Rizzo; Enoch Brandão de Souza Meira-Junior; Ana Márcia S Guimarães; Lilian Gregory; Jorge Timenetsky
Journal:  Braz J Microbiol       Date:  2017-02-20       Impact factor: 2.476

4.  Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4.

Authors:  Manoel N Santos-Junior; Izadora S Rezende; Clarissa L S Souza; Maysa S Barbosa; Guilherme B Campos; Laís F Brito; Éllunny C Queiroz; Elaine N Barbosa; Mariana M Teixeira; Letícia O Da Silva; Lucas S C Silva; Flávia S Nascimento; Tassyo L Da Silva; Adam A Martens; Adriano F P Siqueira; Mayra E O D'Avila Assumpção; Glaucia M Machado-Santelli; Bruno L Bastos; Ana M S Guimarães; Jorge Timenetsky; Lucas M Marques
Journal:  Front Microbiol       Date:  2018-07-12       Impact factor: 5.640

5.  Ureaplasma diversum Genome Provides New Insights about the Interaction of the Surface Molecules of This Bacterium with the Host.

Authors:  Lucas M Marques; Izadora S Rezende; Maysa S Barbosa; Ana M S Guimarães; Hellen B Martins; Guilherme B Campos; Naíla C do Nascimento; Andrea P Dos Santos; Aline T Amorim; Verena M Santos; Sávio T Farias; Fernanda  C Barrence; Lauro M de Souza; Melissa Buzinhani; Victor E Arana-Chavez; Maria E Zenteno; Gustavo P Amarante-Mendes; Joanne B Messick; Jorge Timenetsky
Journal:  PLoS One       Date:  2016-09-07       Impact factor: 3.240
  5 in total

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