Literature DB >> 16614904

Differential gene expression of human CD34+ hematopoietic stem and progenitor cells before and after culture.

Qunliang Li1, Haibo Cai, Qiwei Liu, Wen-Song Tan.   

Abstract

Ex vivo expanded CD34+ hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34+ cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34+ HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.

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Year:  2006        PMID： 16614904     DOI： 10.1007/s10529-005-6064-4

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  2 in total

1.  Cord Blood-Derived Quiescent CD34+ Cells Are More Transcriptionally Matched to AML Blasts Than Cytokine-Induced Normal Human Hematopoietic CD34+ Cells.

Authors:  Richard L Darley; Alex Tonks; Chinmay Munje; Robert K Hills; Anthony Whetton; Alan K Burnett
Journal:  Gene Expr       Date:  2015

2.  A comparative gene-expression analysis of CD34+ hematopoietic stem and progenitor cells grown in static and stirred culture systems.

Authors:  Qunliang Li; Qiwei Liu; Haibo Cai; Wen-Song Tan
Journal:  Cell Mol Biol Lett       Date:  2006-09-14       Impact factor: 5.787
  2 in total

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