A Ozkan1, K Fiskin. 1. Akdeniz University, Art-Science Faculty, Biology Department, Antalya, Turkey. aysunozkan95@yahoo.com
Abstract
AIM: To evaluate protective effect of antioxidant enzymes against epirubicin-HCI (EPI) cytotoxicity in vitro. MATERIALS AND METHODS: Viability of MCF-7 cells treated with EPI was measured using the MTT test. Glutathione (GSH), protein content and enzymatic activity were measured spectrophotometrically. NADPH - dependent cytochrome P-450 reductase (NADPH-CYP-450) and glutathione S-transferase pi (GST-pi) expression in MCF-7 cells were determined by Western blot analysis. RESULTS: The IC50 values of EPI in MCF-7 cells were 1.0, 0.7 and 0.5 ng/ml respectively for 24, 48 and 72 h applications. Simultaneously enzymatic activity of glutathione S-transferase, glutathione peroxidase, GSH and expression of GST-pi, NADPH-CYP-450 reductase were increased in EPI (1 ng/ml) - treated cells at the end of the 24 h incubation. Addition of superoxide dismutase, catalase and GSH decreased cytotoxicity of EPI. CONCLUSION: We hypothesized that the production of reactive oxygen species and hydrogen peroxide as result of EPI treatment can cause cytotoxicity in MCF-7 cells and antioxidant enzymes protect the cells against this process.
AIM: To evaluate protective effect of antioxidant enzymes against epirubicin-HCI (EPI) cytotoxicity in vitro. MATERIALS AND METHODS: Viability of MCF-7 cells treated with EPI was measured using the MTT test. Glutathione (GSH), protein content and enzymatic activity were measured spectrophotometrically. NADPH - dependent cytochrome P-450 reductase (NADPH-CYP-450) and glutathione S-transferase pi (GST-pi) expression in MCF-7 cells were determined by Western blot analysis. RESULTS: The IC50 values of EPI in MCF-7 cells were 1.0, 0.7 and 0.5 ng/ml respectively for 24, 48 and 72 h applications. Simultaneously enzymatic activity of glutathione S-transferase, glutathione peroxidase, GSH and expression of GST-pi, NADPH-CYP-450 reductase were increased in EPI (1 ng/ml) - treated cells at the end of the 24 h incubation. Addition of superoxide dismutase, catalase and GSH decreased cytotoxicity of EPI. CONCLUSION: We hypothesized that the production of reactive oxygen species and hydrogen peroxide as result of EPI treatment can cause cytotoxicity in MCF-7 cells and antioxidant enzymes protect the cells against this process.
Authors: Camila Leonel; Gabriela B Gelaleti; Bruna V Jardim; Marina G Moschetta; Vitor R Regiani; Juliana G Oliveira; Debora Apc Zuccari Journal: BMC Vet Res Date: 2014-02-24 Impact factor: 2.741
Authors: Russell J Needham; Carlos Sanchez-Cano; Xin Zhang; Isolda Romero-Canelón; Abraha Habtemariam; Margaret S Cooper; Levente Meszaros; Guy J Clarkson; Philip J Blower; Peter J Sadler Journal: Angew Chem Int Ed Engl Date: 2016-12-21 Impact factor: 15.336