S Mirza1, J P Jeannon, J Soames, J A Wilson. 1. Department of Otolaryngology-Head & Neck Surgery, Freeman University Hospital, Newcastle-upon-Tyne, UK. showkatmirza@hotmail.com
Abstract
CONCLUSION: Ki67 is not a reliable marker of malignant transformation in laryngeal dysplasia. OBJECTIVES: No reliable means of predicting which cases of laryngeal dysplasia will undergo malignant transformation currently exists. Our aim was to evaluate Ki67, a marker of cell proliferation, as a potential marker for the transformation of laryngeal dysplasia to squamous cell carcinoma. PATIENTS AND METHODS: Eighty consecutive cases of previously untreated patients with a histological diagnosis of laryngeal dysplasia from 1987 to 1993 were identified from the pathological archives. Standard immunohistochemical techniques were used to identify Ki67-positive cells and activity was scored on a scale of 0-4 using defined criteria. RESULTS: Of the 80 cases there were 24 females and 56 males with a mean age of 56 years (range 29-80 years). Twenty cases subsequently transformed to a squamous cell carcinoma. For each Ki67 score (0-4), the rate of malignant transformation was: 0, 1 of 6 patients (17%); 1, 7 of 33 patients (21%); 2, 5 of 22 patients (23%); 3, 4 of 13 patients (31%); and 4, 3 of 6 patients (50%). A higher Ki67 score seemed to correlate with a higher likelihood of malignant transformation but this did not reach statistical significance (p=0.17, Pearson chi(2) test). Considering a score of 3 or 4 as positive for predicting malignant transformation produced a test of relatively high specificity (80%) but poor sensitivity (35%).
CONCLUSION: Ki67 is not a reliable marker of malignant transformation in laryngeal dysplasia. OBJECTIVES: No reliable means of predicting which cases of laryngeal dysplasia will undergo malignant transformation currently exists. Our aim was to evaluate Ki67, a marker of cell proliferation, as a potential marker for the transformation of laryngeal dysplasia to squamous cell carcinoma. PATIENTS AND METHODS: Eighty consecutive cases of previously untreated patients with a histological diagnosis of laryngeal dysplasia from 1987 to 1993 were identified from the pathological archives. Standard immunohistochemical techniques were used to identify Ki67-positive cells and activity was scored on a scale of 0-4 using defined criteria. RESULTS: Of the 80 cases there were 24 females and 56 males with a mean age of 56 years (range 29-80 years). Twenty cases subsequently transformed to a squamous cell carcinoma. For each Ki67 score (0-4), the rate of malignant transformation was: 0, 1 of 6 patients (17%); 1, 7 of 33 patients (21%); 2, 5 of 22 patients (23%); 3, 4 of 13 patients (31%); and 4, 3 of 6 patients (50%). A higher Ki67 score seemed to correlate with a higher likelihood of malignant transformation but this did not reach statistical significance (p=0.17, Pearson chi(2) test). Considering a score of 3 or 4 as positive for predicting malignant transformation produced a test of relatively high specificity (80%) but poor sensitivity (35%).
Authors: Juan P Rodrigo; Juana María García-Pedrero; Carlos Suárez; Robert P Takes; Lester D R Thompson; Pieter J Slootweg; Julia A Woolgar; William H Westra; Ruud H Brakenhoff; Alessandra Rinaldo; Kenneth O Devaney; Michelle D Williams; Douglas R Gnepp; Alfio Ferlito Journal: Eur Arch Otorhinolaryngol Date: 2011-11-12 Impact factor: 2.503
Authors: Fernando López; César Alvarez-Marcos; Marta Alonso-Guervós; Francisco Domínguez; Carlos Suárez; Mario A Hermsen; José L Llorente Journal: Eur Arch Otorhinolaryngol Date: 2013-04-07 Impact factor: 2.503