PURPOSE: We examined the effect of Glubran2 on the rat urethra and spongiosal tissue as well as the histopathological effect. We also investigated its clinical use. MATERIALS AND METHODS: Eight control and 12 study group male Wistar rats were used in our study. The corpus spongiosum tissues, including the urethra and the tunica albuginea of the testis, were cut 0.5 and 0.2 cm long, respectively. Incision areas in the control group were sutured. In the study group Glubran2 was dripped on the area. Three weeks later all rats were sacrificed. The penile and testicular tissues were histopathologically investigated. One-way ANOVA and the t test were applied for statistical analysis. RESULTS: When the study group was compared with the control group, the difference in urethral healing (p = 0.001), urethral lumen irregularity (p = 0.036), spongiosal tissue healing (p = 0.001), inflammation (p = 0.000), hyperemia-bleeding (p = 0.036) and total healing (p = 0.001) were statistically significant. When the study and control groups were compared, the difference in inflammation (p = 0.000), necrotic areas (p = 0.002), tunica albuginea irregularity (p = 0.005), Sertoli's and Leydig cell destruction (p = 0.005), and testicular spermatogenesis (p = 0.005) were statistically significant. CONCLUSIONS: While perfect healing in the urethral and spongiosal tissues supports the clinical application, damage to the seminiferous tubules, decreased spermatogenesis at the operated site, tunica albuginea irregularity in a fourth of the testes and calcification in 1 testis were observed.
PURPOSE: We examined the effect of Glubran2 on the rat urethra and spongiosal tissue as well as the histopathological effect. We also investigated its clinical use. MATERIALS AND METHODS: Eight control and 12 study group male Wistar rats were used in our study. The corpus spongiosum tissues, including the urethra and the tunica albuginea of the testis, were cut 0.5 and 0.2 cm long, respectively. Incision areas in the control group were sutured. In the study group Glubran2 was dripped on the area. Three weeks later all rats were sacrificed. The penile and testicular tissues were histopathologically investigated. One-way ANOVA and the t test were applied for statistical analysis. RESULTS: When the study group was compared with the control group, the difference in urethral healing (p = 0.001), urethral lumen irregularity (p = 0.036), spongiosal tissue healing (p = 0.001), inflammation (p = 0.000), hyperemia-bleeding (p = 0.036) and total healing (p = 0.001) were statistically significant. When the study and control groups were compared, the difference in inflammation (p = 0.000), necrotic areas (p = 0.002), tunica albuginea irregularity (p = 0.005), Sertoli's and Leydig cell destruction (p = 0.005), and testicular spermatogenesis (p = 0.005) were statistically significant. CONCLUSIONS: While perfect healing in the urethral and spongiosal tissues supports the clinical application, damage to the seminiferous tubules, decreased spermatogenesis at the operated site, tunica albuginea irregularity in a fourth of the testes and calcification in 1 testis were observed.
Authors: Gabriela Ambriz-González; Pedro Aguirre-Ramirez; José Manuel García-de León; Francisco Javier León-Frutos; Sergio Adrián Montero-Cruz; Xóchitl Trujillo; Clotilde Fuentes-Orozco; Michel Dassaejv Macías-Amezcua; Andrea del Socorro Álvarez-Villaseñor; Ana Olivia Cortés-Flores; Mariana Chávez-Tostado; Alejandro González-Ojeda Journal: BMC Urol Date: 2014-11-21 Impact factor: 2.264