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Literature DB >> 16595249

Increased ammonium in culture medium reduces the development of human embryos to the blastocyst stage.

Irma Virant-Klun¹, Tomaz Tomazevic, Eda Vrtacnik-Bokal, Andrej Vogler, Mladen Krsnik, Helena Meden-Vrtovec.

Abstract

The objective of this prospective study was to evaluate the effect of ammonium accumulated in sequential media and determined by enzymatic spectrophotometric method on the blastocyst development in 281 human embryos from 100 stimulated and natural in vitro fertilization (IVF) cycles. Ammonium concentration was increased in 62% of cycles and was correlated negatively with the blastocyst development after classical IVF, but not after intracytoplasmic sperm injection (ICSI).

Entities: Chemical Disease Species

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Year: 2006 PMID： 16595249 DOI： 10.1016/j.fertnstert.2005.10.018

Source DB: PubMed Journal: Fertil Steril ISSN： 0015-0282 Impact factor: 7.329

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Cited

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4. Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes.

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Increased ammonium in culture medium reduces the development of human embryos to the blastocyst stage.

1. Reduced blastocyst formation in reduced culture volume.

2. When stresses collide.

3. An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments.

4. Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes.

5. A microwell culture system that allows group culture and is compatible with human single media.

6. Effect of Dipeptides on In vitro Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes.

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8. Concerns about the paper "A descriptive study of culture media in Brazilian assisted reproduction clinics".