| Literature DB >> 16594057 |
T Caspar1, T P Lin, J Monroe, W Bernhard, S Spilatro, J Preiss, C Somerville.
Abstract
Three classes of mutants of Arabidopsis thaliana (L.) Heynhold with alterations in starch metabolism were found to have higher levels of leaf amylase activity than the wild type when grown in a 12-hr photoperiod. This effect was dependent upon the developmental stage of the plants and was largely suppressed during growth in continuous light. The various amylolytic activities in crude extracts were separated by electrophoresis in nondenaturing polyacrylamide gels and visualized by activity staining. The increased amylase activity in the mutants was due to an up to 40-fold increase in the activity of an extrachloroplast beta-amylase (EC 3.2.1.2). These observations indicate the existence of a regulatory mechanism that controls the amount of beta-amylase activity in response to fluctuations in photosynthetic carbohydrate metabolism. It is paradoxical that beta-amylase appears to be a highly regulated enzyme, but as yet no physiologically relevant function can be assigned to this enzyme due to the absence of starch in the cytoplasmic compartment of leaf cells.Entities:
Year: 1989 PMID： 16594057 PMCID： PMC297724 DOI： 10.1073/pnas.86.15.5830
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205