Literature DB >> 16593793

Expression and assembly of functional bacterial luciferase in plants.

C Koncz1, O Olsson, W H Langridge, J Schell, A A Szalay.   

Abstract

The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal,reduced FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium-mediated or direct DNA transformation. Simultaneous expression of the luxA and luxB genes was monitored by protein immunoblot analysis. Luciferase-mediated light emission provided evidence for the assembly of the two protein subunits into a functional dimeric enzyme in plant protoplasts, in transformed calli, and in leaves of transformed plants. Bacterial luciferase may provide a useful marker-gene system for the quantitative assay of coordinate gene expression in transgenic plants.

Entities:  

Year:  1987        PMID： 16593793      PMCID： PMC304156          DOI： 10.1073/pnas.84.1.131

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Bacterial bioluminescence: isolation and expression of the luciferase genes from Vibrio harveyi.

Authors:  R Belas; A Mileham; D Cohn; M Hilman; M Simon; M Silverman
Journal:  Science       Date:  1982-11-19       Impact factor: 47.728

2.  Selection-expression plasmid vectors for use in genetic transformation of higher plants.

Authors:  J Velten; J Schell
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

3.  Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the alpha subunit of bacterial luciferase.

Authors:  D H Cohn; A J Mileham; M I Simon; K H Nealson; S K Rausch; D Bonam; T O Baldwin
Journal:  J Biol Chem       Date:  1985-05-25       Impact factor: 5.157

4.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469

5.  Cloning of the luciferase structural genes from Vibrio harveyi and expression of bioluminescence in Escherichia coli.

Authors:  T O Baldwin; T Berends; T A Bunch; T F Holzman; S K Rausch; L Shamansky; M L Treat; M M Ziegler
Journal:  Biochemistry       Date:  1984-07-31       Impact factor: 3.162

6.  Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.

Authors:  J R de Wet; K V Wood; D R Helinski; M DeLuca
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

7.  A simple and general method for transferring genes into plants.

Authors: 
Journal:  Science       Date:  1985-03-08       Impact factor: 47.728

8.  Chimeric genes as dominant selectable markers in plant cells.

Authors:  L Herrera-Estrella; M D Block; E Messens; J P Hernalsteens; M V Montagu; J Schell
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

9.  Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens.

Authors:  J Velten; L Velten; R Hain; J Schell
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598

10.  Protein fusions with the kanamycin resistance gene from transposon Tn5.

Authors:  B Reiss; R Sprengel; H Schaller
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598
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  25 in total

1.  Control of excision frequency of maize transposable element Ds in Petunia protoplasts.

Authors:  D Becker; R Lütticke; M Li; P Starlinger
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

2.  High-frequency T-DNA-mediated gene tagging in plants.

Authors:  C Koncz; N Martini; R Mayerhofer; Z Koncz-Kalman; H Körber; G P Redei; J Schell
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

Review 3.  Molecular biology of bacterial bioluminescence.

Authors:  E A Meighen
Journal:  Microbiol Rev       Date:  1991-03

4.  Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana.

Authors:  R S Sangwan; Y Bourgeois; S Brown; G Vasseur; B Sangwan-Norreel
Journal:  Planta       Date:  1992-10       Impact factor: 4.116

5.  A plant transformation vector with a minimal T-DNA II. Irregular integration patterns of the T-DNA in the plant genome.

Authors:  P Porsch; A Jahnke; K Düring
Journal:  Plant Mol Biol       Date:  1998-06       Impact factor: 4.076

6.  Excision of a Ds-like maize transposable element (Ac delta) in a transient assay in Petunia is enhanced by a truncated coding region of the transposable element Ac.

Authors:  N Houba-Hérin; D Becker; A Post; Y Larondelle; P Starlinger
Journal:  Mol Gen Genet       Date:  1990-10

7.  The use of the luxA gene of the bacterial luciferase operon as a reporter gene.

Authors:  O Olsson; C Koncz; A A Szalay
Journal:  Mol Gen Genet       Date:  1988-12

8.  Enhanced stable expression of aVibrio luciferase under the control of the Ω-translational enhancer in transgenic plants.

Authors:  K Okumura; L Chlumsky; T O Baldwin; C I Kado
Journal:  World J Microbiol Biotechnol       Date:  1992-11       Impact factor: 3.312

9.  Altered Growth and Wood Characteristics in Transgenic Hybrid Aspen Expressing Agrobacterium tumefaciens T-DNA Indoleacetic Acid-Biosynthetic Genes.

Authors:  H. Tuominen; F. Sitbon; C. Jacobsson; G. Sandberg; O. Olsson; B. Sundberg
Journal:  Plant Physiol       Date:  1995-12       Impact factor: 8.340

10.  Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line.

Authors:  Dan M Close; Stacey S Patterson; Steven Ripp; Seung J Baek; John Sanseverino; Gary S Sayler
Journal:  PLoS One       Date:  2010-08-27       Impact factor: 3.240
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