Literature DB >> 16593305

Theory of measurement of Förster-type energy transfer in macromolecules.

B Epe1, K G Steinhäuser, P Woolley.   

Abstract

We describe the theoretical basis of an unconventional method for the determination of the amount of energy transferred between two fluorophores by the Förster mechanism. The method involves an internal comparison made by separation of the fluorophores in situ (i.e., in the optical cell), for example, by means of enzymic digestion; it eliminates several important sources of error and it simplifies calculation while making maximal use of the information contained in the fluorescence spectra. The validity of the method is demonstrated by determination of the known distance between two modifiable sites on the transfer RNA molecule, and its usefulness is exemplified by its application to triangulation of the ribosome of Escherichia coli.

Entities:  

Year:  1983        PMID： 16593305      PMCID： PMC393869          DOI： 10.1073/pnas.80.9.2579

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  Use of substrates with fluorescent donor and acceptor chromophores for the kinetic assay of hydrolases.

Authors:  Amos Carmel; Margalit Zur; Arieh Yaron; Ephraim Katchalski
Journal:  FEBS Lett       Date:  1973-02-15       Impact factor: 4.124

2.  Structure of yeast phenylalanine tRNA at 3 A resolution.

Authors:  J D Robertus; J E Ladner; J T Finch; D Rhodes; R S Brown; B F Clark; A Klug
Journal:  Nature       Date:  1974-08-16       Impact factor: 49.962

3.  Ribosomal proteins. Protein compositions of biosynthetic precursors and artifical subparticles from ribosomal subunits in Escherichia coli K 12.

Authors:  H E Homann; K H Nierhaus
Journal:  Eur J Biochem       Date:  1971-05-28

4.  Distances between 3' ends of ribosomal ribonucleic acids reassembled into Escherichia coli ribosomes.

Authors:  O W Odom; D J Robbins; J Lynch; D Dottavio-Martin; G Kramer; B Hardesty
Journal:  Biochemistry       Date:  1980-12-23       Impact factor: 3.162

5.  Distance measurement by energy transfer: the 3' end of 16-S RNA and proteins S4 and S17 of the ribosome of Escherichia coli.

Authors:  B Epe; P Woolley; K G Steinhäuser; J Littlechild
Journal:  Eur J Biochem       Date:  1982-12

6.  Thin-layer chromatography of oligonucleotides: a device to aid the ultraviolet detection of fingerprint patterns.

Authors:  L Steinhäuser; P Woolley; K Friedrich
Journal:  Anal Biochem       Date:  1982-02       Impact factor: 3.365

7.  Structure of ribosomal protein L6 from Escherichia coli. A fluorescence study.

Authors:  K G Steinhäuser; P Woolley; B Epe; J Dijk
Journal:  Eur J Biochem       Date:  1982-10

8.  Effect of the orientation of donor and acceptor on the probability of energy transfer involving electronic transitions of mixed polarization.

Authors:  E Haas; E Katchalski-Katzir; I Z Steinberg
Journal:  Biochemistry       Date:  1978-11-14       Impact factor: 3.162

9.  Intramolecularly-quenched fluorescent peptides as fluorogenic substrates ofleucine aminopeptidase and inhibitors of clostridial aminopeptidase.

Authors:  A Carmel; E Kessler; A Yaron
Journal:  Eur J Biochem       Date:  1977-03-01

10.  Studies of transfer RNA tertiary structure of singlet-singlet energy transfer.

Authors:  C H Yang; D Söll
Journal:  Proc Natl Acad Sci U S A       Date:  1974-07       Impact factor: 11.205
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  15 in total

1.  Catalysis of peptide dissociation from class II MHC-peptide complexes.

Authors:  L Schmitt; J R Kratz; M M Davis; H M McConnell
Journal:  Proc Natl Acad Sci U S A       Date:  1999-06-08       Impact factor: 11.205

2.  DsRed as a potential FRET partner with CFP and GFP.

Authors:  Michael G Erickson; Daniel L Moon; David T Yue
Journal:  Biophys J       Date:  2003-07       Impact factor: 4.033

3.  Probing the conformation of the fibronectin III1-2 domain by fluorescence resonance energy transfer.

Authors:  Nancy W Karuri; Zong Lin; Hays S Rye; Jean E Schwarzbauer
Journal:  J Biol Chem       Date:  2008-12-08       Impact factor: 5.157

4.  FRET spectrometry: a new tool for the determination of protein quaternary structure in living cells.

Authors:  Valerică Raicu; Deo R Singh
Journal:  Biophys J       Date:  2013-11-05       Impact factor: 4.033

5.  Experimental verification of the kinetic theory of FRET using optical microspectroscopy and obligate oligomers.

Authors:  Suparna Patowary; Luca F Pisterzi; Gabriel Biener; Jessica D Holz; Julie A Oliver; James W Wells; Valerică Raicu
Journal:  Biophys J       Date:  2015-04-07       Impact factor: 4.033

6.  Identification of a membrane-targeting domain of the transient receptor potential canonical (TRPC)4 channel unrelated to its formation of a tetrameric structure.

Authors:  Jongyun Myeong; Misun Kwak; Chansik Hong; Ju-Hong Jeon; Insuk So
Journal:  J Biol Chem       Date:  2014-10-27       Impact factor: 5.157

7.  Fluorimetric distance determination by resonance energy transfer. Ribosome-bound transfer RNA.

Authors:  K Friedrich; P Woolley; K G Steinhäuser
Journal:  Eur Biophys J       Date:  1988       Impact factor: 1.733

8.  Structural changes in subdomain 2 of G-actin observed by fluorescence spectroscopy.

Authors:  J Moraczewska; H Strzelecka-Gołaszewska; P D Moens; C G dos Remedios
Journal:  Biochem J       Date:  1996-07-15       Impact factor: 3.857

9.  OS-FRET: a new one-sample method for improved FRET measurements.

Authors:  Annette H Erbse; Adam J Berlinberg; Ching-Ying Cheung; Wai-Yee Leung; Joseph J Falke
Journal:  Biochemistry       Date:  2010-12-30       Impact factor: 3.162

10.  Protein interaction quantified in vivo by spectrally resolved fluorescence resonance energy transfer.

Authors:  Valerică Raicu; David B Jansma; R J Dwayne Miller; James D Friesen
Journal:  Biochem J       Date:  2005-01-01       Impact factor: 3.857
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